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008			230605s2022 xxu| o |||| 0|eng d
020			_a9781071623251
024	7		_a10.1007/978-1-0716-2325-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2022 EB
245	0	0	_aProteoform Identification : _bMethods and Protocols _cedited by Liangliang Sun, Xiaowen Liu
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (X, 240 páginas) _b82 ilustraciones, 74 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2500
505	0		_aProteoforms and Proteoform Families: Past, Present, and Future -- Membrane Ultrafiltration-Based Sample Preparation Method and Sheath-Flow CZE-MS/MS for Top-Down Proteomics -- Size Exclusion Chromatography Strategies and MASH Explorer for Large Proteoform Characterization -- RPLC-RPLC-MS/MS for Proteoform Identification -- Monolithic Materials-Based RPLC-MS for Proteoform Separation and Identification -- Capillary Isoelectric Focusing: Mass Spectrometry Method for the Separation and Online Characterization of Monoclonal Antibody Charge Variants at Intact and Subunit Level -- Proteoform Analysis and Construction of Proteoform Families in Proteoform Suite -- Top-Down Mass Spectrometry Data Analysis Using TopPIC Suite -- Accurate Proteoform Identification and Quantitation Using pTop 2.0 -- Proteoform Identification and Quantification using Intact Protein Database Search Engine ProteinGoggle -- Mass Deconvolution of Top-Down Mass Spectrometry Datasets by FLASHDeconv -- Deconvolving Native and Intact Protein Mass Spectra with UniDec -- Discovery of Unknown Post-Translational Modifications by Top-Down Mass Spectrometry -- Determining Copper and Zinc Content in Superoxide Dismutase using Electron Capture Dissociation under Native Spray Conditions -- Surface-Induced Dissociation for Protein Complex Characterization.
520			_aThis volume discusses the latest mass spectrometry (MS)-based technologies for proteoform identification, characterization, and quantification. Some of the topics covered in this book include sample preparation, proteoform separation, proteoform gas-phase fragmentation, and bioinformatics tools for MS data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Proteoform Identification: Methods and Protocols is a valuable resource for researchers in both academia and the biopharmaceutical industry who are interested in proteoform analysis using MS. .
988			_aSpringer_Protocols_2022
650		7	_2embne _9430647 _aProteómica
776	0	8	_iPrinted edition: _z9781071623244
776	0	8	_iPrinted edition: _z9781071623268
776	0	8	_iPrinted edition: _z9781071623275
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2325-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b05/2023 _dz _eIG _zSI