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008			230506s2022 xxu| o |||| 0|eng d
020			_a9781071622216
024	7		_a10.1007/978-1-0716-2221-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH600 _b2022 EB
245	0	0	_aChromosome Architecture : _bMethods and Protocols _cedited by Mark C. Leake
250			_a2nd edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 330 páginas) _b85 ilustraciones, 76 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2476
505	0		_aA Next Generation of Advances in Chromosome Architecture -- Single Molecule Narrowfield Microscopy of Protein-DNA Binding Dynamics in Glucose Signal Transduction of Live Yeast Cells -- Convolutional Neural Networks for Classifying Chromatin Morphology in Live Cell Imaging -- Fluorescence Recovery After Photobleaching (FRAP) to Study Dynamics of the Structural Maintenance of Chromosome (SMC) Complex in Live Escherichia coli Bacteria -- Atomic Force Microscopy of DNA and DNA-Protein Interactions -- Live-Cell Visualization of DNA Transfer and Pilus Dynamics during Bacterial Conjugation -- Transverse Magnetic Tweezers Allowing Coincident Epi-Fluorescence Microscopy on Horizontally Extended DNA -- Atomistic Molecular Dynamics Simulations of DNA in Complex 3D Arrangements for Comparison with Lower Resolution Structural Experiments -- Replication Labeling Methods for Super-Resolution Imaging of Chromosome Territories and Chromatin Domains -- DNA-Protein Interactions Studied Directly Using iSCAT Imaging of GNP-Tagged Proteins -- Escherichia coli Chromosome Copy Number Measurement Using Flow Cytometry Analysis -- Dynamics of Bacterial Chromosomes by Locus Tracking in Fluorescence Microscopy -- Measuring Nuclear Mechanics with Atomic Force Microscopy -- Copy Number Analysis of the Yeast Histone Deacetylase Complex Component Cti6 Directly in Living Cells -- Super-Resolution Microscopy and Tracking of DNA-Binding Proteins in Bacterial Cells -- Studying the Dynamics of Chromatin-Binding Proteins in Mammalian Cells Using Single-Molecule Localization Microscopy -- The End Restraint Method for Mechanically Perturbing Nucleic Acids In Silico -- Visualizing the Replisome, Chromosome Breaks, and Replication Restart in Bacillus subtilis -- High-Throughput Imaging of Bacillus subtilis -- Measuring Nuclear Organization of Proteins with STORM Imaging and Cluster Analysis -- Single-Molecular Quantification of Flowering Control Proteins within Nuclear Condensates in Live Whole Arabidopsis Root.
520			_aThis detailed new edition collects cutting-edge laboratory protocols, techniques, and applications in use by some of the leading international experts in the broad field of chromosome architecture. The book emphasizes the increasing physiological relevance of chromosome architecture investigation, manifest both through application of more complex bottom-up assays in vitro as well as through maintaining the native physiological context through the investigation of living, functional cells. In addition, the chapters reflect the dramatic improvement in the length scale of precision by utilizing single-molecule approaches, both for imaging the DNA content of chromosome and proteins that bind to DNA as well as using methods that can controllably manipulate single DNA molecules, and the use of advanced computational methods and mathematical analysis is also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Chromosome Architecture: Methods and Protocols, Second Edition is an ideal guide for researchers working in this dynamic area of study.
988			_aSpringer_Protocols_2022
650		7	_2embne _9139992 _aCromosomas
650		7	_2embne _9140562 _aGenética humana
776	0	8	_iPrinted edition: _z9781071622209
776	0	8	_iPrinted edition: _z9781071622223
776	0	8	_iPrinted edition: _z9781071622230
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2221-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b06/2023 _dz _eIG _zSI