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008			230605s2022 xxu| o |||| 0|eng d
020			_a9781071622414
024	7		_a10.1007/978-1-0716-2241-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK981.5 _b2022 EB
245	0	0	_aRecombinant Proteins in Plants : _bMethods and Protocols _cedited by Stefan Schillberg, Holger Spiegel
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (X, 346 páginas) _b51 ilustraciones, 39 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2480
505	0		_aRecombinant Protein Production in Plants - A Brief Overview of Strengths and Challenges -- Production of Recombinant Proteins in Transgenic Tobacco Plants -- Molecular Farming in Seed Crops: Gene Transfer into Barley (Hordeum vulgare) and wheat (Triticum aestivum) -- Cell Biology Methods to Study Recombinant Proteins in Seeds -- Production of Recombinant Glycoproteins in Nicotiana tabacum BY-2 Suspension Cells -- Production of Recombinant Proteins by Agrobacterium-mediated Transient Expression -- Specific packaging of custom RNA molecules into Cowpea Mosaic Virus-Like Particles -- Plant-based Cell-Free In Vitro Transcription and Translation of Recombinant Proteins -- Strategies for Efficient and Sustainable Protein Extraction and Purification from Plant Tissues -- Improving Recombinant Protein Recovery from Plant Tissue Using Heat Precipitation -- Techno-economic Modeling and Simulation for Plant-Based Manufacturing of Recombinant Proteins -- Optimization of Vectors and Targeting Strategies Including Goldenbraid and Genome Editing Tools -GoldenBraid assembly of multiplex CRISPR/Cas12a guide RNAs for Gene Editing in Nicotiana benthamiana -- Advanced Fusion Strategies for The Production Of Functionalized Potato Virus X virions -- Knockout of Glycosyltransferases in Nicotiana benthamiana by Genome Editing to Improve Glycosylation of Plant-Produced Proteins -- A bioluminescent Agrobacterium tumefaciens for Imaging Bacterial Metabolic Activity in planta -- Statistical Designs to Improve Downstream Processing -- Regulation of Molecular Farming Products -- Freedom to Operate Analysis of Molecular Farming Projects.
520			_aThis volume provided methods and protocols on recombinant protein production in different plant systems, downstream processing, and strategies to optimize protein expression. Chapters guide readers through recombinant protein production in important plant systems, protein recovery and purification, different strategies to optimise productivity, cloning and fusion protein approaches, and the regulation and freedom to operate analysis of plant-produced proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Proteins in Plants: Methods and Protocols aims to be useful to newcomers and experienced researchers interested in expanding their expertise in the field of plant-based protein production. Chapters 6, 8 and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
988			_aSpringer_Protocols_2022
650		7	_2embne _9140814 _aADN recombinante
650		7	_2embne _9139640 _aGenética vegetal
776	0	8	_iPrinted edition: _z9781071622407
776	0	8	_iPrinted edition: _z9781071622421
776	0	8	_iPrinted edition: _z9781071622438
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2241-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eIG _zSI