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008			230605s2022 xxu| o |||| 0|eng d
020			_a9781071619711
024	7		_a10.1007/978-1-0716-1971-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR201 .B34 _b2022 EB
245	0	0	_aBacterial Virulence : _bMethods and Protocols _cedited by Ohad Gal-Mor
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XII, 267 páginas) _b71 ilustraciones, 53 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2427
505	0		_aGeneration of Markerless Gene Deletion Mutants in Listeria monocytogenes Using a Mutated PheS for Counterselection -- A Rapid Fluorescence-Based Screen to Identify Regulators and Components of Interbacterial Competition Mechanisms in Bacteria -- Predicting Type III Effector Proteins Using the Effectidor Web-Server -- Assay for Type III Secretion in Escherichia coli -- Profiling of Secreted Type 3 Secretion System Substrates by Salmonella enterica -- Analysis of SPI-1 Dependent Type III Secretion and Injection Using a NanoLuc Luciferase-Based Assay -- Mycobacterium tuberculosis Infection of THP-1 Cells: A Model for High Content Analysis of Intracellular Growth and Drug Susceptibility -- Bone Marrow-Derived Macrophage (BMDM) Infection by Listeria monocytogenes -- Preparation and Inflammasome Activation of Murine Bone Marrow-Derived and Resident Peritoneal Macrophages -- Flow Cytometry-Based Single Cell Analyses of Bacterial Adaption to Intracellular Environments -- Quantification of Microbial Fluorescent Sensors during Live Intracellular Infections -- Dissecting Human Blood Immune Cells Response to Intracellular Infection Using Single Cell RNA-Sequencing -- Salmonella enterica Infection of Human and Mouse Colon Organoid-Derived Monolayers -- Analysis of Salmonella enterica Adhesion to Leaves of Corn Salad or Lettuce -- Methods for Using the Galleria mellonella Invertebrate Model to Probe Enterococcus faecalis Pathogenicity -- Murine Soft Tissue Infection Model to Study Group A Streptococcus (GAS) Pathogenesis in Necrotizing Fasciitis -- Mouse Model to Study Salmonella-Induced Colitis -- Analysis of Salmonella Typhi Pathogenesis in a Humanized Mouse Model -- In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella -- Two In Vivo Models to Study Salmonella Asymptomatic Carrier State in Chicks.
520			_aThis detailed volume explores the field of bacterial virulence and the effort to understand how microbial interaction with a host results in the pathology of a specific disease. This collection of selected protocols includes advanced molecular biology and bioinformatics methods, cell culture and organoid models of infection, as well as in vivo infection models that are useful to study the interaction of pathogens with plants, insects, avian, and mammalian hosts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and beneficial, Bacterial Virulence: Methods and Protocols serves as an ideal guide for researchers seeking to promote and further develop the exciting and continuously evolving field of bacterial virulence. Chapter 19 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
988			_aSpringer_Protocols_2022
650		7	_2embne _9146593 _aEnfermedades bacterianas
650		7	_2embne _9138684 _aMicrobiología
650		7	_2embne _9673629 _aRelaciones huesped bacteria
776	0	8	_iPrinted edition: _z9781071619704
776	0	8	_iPrinted edition: _z9781071619728
776	0	8	_iPrinted edition: _z9781071619735
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1971-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eIG _zSI