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020 _a9781071621240
024 7 _a10.1007/978-1-0716-2124-0
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP551
_b2022 EB
245 0 0 _aProteomics in Systems Biology :
_bMethods and Protocols
_cedited by Jennifer Geddes-McAlister
250 _a1st edition 2022
264 1 _aNew York, NY
_bSpringer International Publising
_c2022
300 _a1 recurso en línea (XII, 370 páginas)
_b55 ilustraciones, 53 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2456
505 0 _aReview of the Real and Sometimes Hidden Costs in Proteomics Experimental Workflows -- High-Throughput Mass Spectrometry-Based Proteomics with dia-PASEF -- Isolation of Detergent Insoluble Proteins from Mouse Brain Tissue for Quantitative Analysis Using Data Independent Acquisition (DIA) -- Rodent Lung Tissue Sample Preparation and Processing for Shotgun Proteomics -- Protein Purification and Digestion Methods for Bacterial Proteomic Analyses -- Mapping Cell Surface Proteolysis with Plasma Membrane-Targeted Subtiligase -- N-Terminomics/TAILS of Tissue and Liquid Biopsies -- HUNTER: Sensitive Automated Characterization of Proteolytic Systems by N Termini Enrichment from Microscale Specimen -- Phosphoproteomics and Organelle Proteomics in Pancreatic Islets -- Phosphoproteomic Sample Preparation for Global Phosphorylation Profiling of a Fungal Pathogen -- Glycopeptide-Centric Approaches for the Characterization of Microbial Glycoproteomes -- Integrated Network Discovery Using Multi-Proteomic Data -- Targeted Cross-Linking Mass Spectrometry on Single-Step Affinity Purified Molecular Complexes in the Yeast Saccharomyces cerevisiae -- A Crosslinking - Mass Spectrometry Protocol for the Structural Analysis of Microtubule-Associated Proteins -- Comprehensive Interactome Mapping of Nuclear Receptors Using Proximity Biotinylation -- Mining Proteomics Datasets to Uncover Functional Pseudogenes -- Proteomic Profiling of the Interplay between a Bacterial Pathogen and Host Uncovers Novel Anti-Virulence Strategies -- Affinity-Enrichment of Salmonella-Modified Membranes from Murine Macrophages for Proteomic Analyses -- Proteomic Profiling of Interplay between Agrobacterium tumefaciens and Nicotiana benthamiana for Improved Molecular Pharming Outcomes -- Label-Free Quantitative Proteomic Profiling of Fusarium Head Blight in Wheat -- DIA Proteomics and Machine Learning for the Fast Identification of Bacterial Species in Biological Samples -- Novel Bioinformatics Strategies Driving Dynamic Metaproteomic Studies -- MaxQuant Module for the Identification of Genomic Variants Propagated into Peptides -- Untargeted Metabolomic Profiling of Fungal Species Populations.
520 _aThis detailed book highlights the diverse techniques and applications of proteomics in an accessible, informative, and concise manner. The collection features sample preparation from distinct extraction, quantification, enrichment, modification, as well as interactome methodology for the in-depth exploration of biological systems, and the application of proteomics to clinical, infectious disease, and agricultural practices. Moreover, cutting-edge bioinformatics tools, encompassing machine learning and data integration strategies, are explored, as are techniques expanding beyond proteomics into the realm of metabolomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting known pitfalls. Comprehensive and practical, Proteomics in Systems Biology: Methods and Protocols emphasizes the importance of proteomics and demonstrates a plethora of approaches for investigating diverse biological entities from a systems perspective.
988 _aSpringer_Protocols_2022
650 7 _2embne
_9139861
_aProteínas
650 7 _2embne
_9403517
_aSistemas biológicos
650 7 _2embne
_9430647
_aProteómica
776 0 8 _iPrinted edition:
_z9781071621233
776 0 8 _iPrinted edition:
_z9781071621257
776 0 8 _iPrinted edition:
_z9781071621264
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2124-0
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b06/2023
_dz
_eIG
_zSI