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008			230603s2022 xxu| o |||| 0|eng d
020			_a9781071616536
024	7		_a10.1007/978-1-0716-1653-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK725 _b2022 EB
245	0	0	_aPlant Mitochondria : _bMethods and Protocols _cedited by Olivier Van Aken, Allan G. Rasmusson
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIII, 342 páginas) _b67 ilustraciones, 49 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2363
505	0		_aPurification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients -- Cell Type-Specific Isolation of Mitochondria in Arabidopsis -- Isolation of Plant Mitochondria Using Affinity Purification -- Isolation of Highly Purified, Intact, and Functional Mitochondria from Potato Tubers Using a Two-in-One Percoll Density Gradient -- Integrity Assessment of Isolated Plant Mitochondria -- High-Throughput Oxygen Consumption Measurements in Leaf Tissue Using Oxygen Sensitive Fluorophores -- Assessment of Respiratory Enzymes in Intact Cells by Permeabilization with Alamethicin -- Assessing the Kinetics of Metabolite Uptake and Utilization by Isolated Mitochondria Using Selective Reaction Monitoring-Mass Spectrometry (SRM-MS) -- Complexome Profiling of Plant Mitochondrial Fractions -- High Throughput BN-PAGE for Mitochondrial Respiratory Complexes -- Assessment of Mitochondrial Protein Composition and Purity by Mass Spectroscopy -- Mitochondrial GFP-Tagged Protein Localization Using Transient Transformations in Arabidopsis thaliana -- Assessment of Mitochondrial Protein Topology and Membrane Insertion -- Assessment of Protein Synthesis in Mitochondria Isolated from Rosette Leaves and Liquid Culture Seedlings of Arabidopsis -- Markers for Mitochondrial ROS Status -- Mass Spectrometry-Based Quantitative Cysteine Redox Proteome Profiling of Isolated Mitochondria Using Differential iodoTMT Labeling -- Analysis of the Plant Mitochondrial Transcriptome -- Quantification of Mitochondrial RNA Editing Efficiency Using Sanger Sequencing Data -- Nascent Transcript Sequencing for the Mapping of Promoters in Arabidopsis thaliana Mitochondria -- Assessment of Mitochondrial DNA Copy Number, Stability, and Repair in Arabidopsis -- Measuring the Activity of DNA Repair Enzymes in Isolated Mitochondria -- MitoTALENs: A Method for Targeted Gene Disruption in Plant Mitochondrial Genomes.
520			_aThis detailed volume presents a wide range of techniques for plant mitochondrial analysis, ranging from tried-and-tested work horse techniques to the latest innovations. Within its pages, it explores subjects such as affinity-based isolation of mitochondria with magnetic beads, mitochondrial quality assessment protocols, measurement of uptake and release of specific metabolites, mitochondrial protein identification and visualization, as well as gene splicing and editing, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Mitochondria: Methods and Protocols provides a highly useful set of methodologies for the plant mitochondrial community to help discover more interesting aspects of plant mitochondria in the years to come.
988			_aSpringer_Protocols_2022
650		7	_2embne _9146302 _aCélulas vegetales
650		7	_2embne _9165668 _aFisiología celular
776	0	8	_iPrinted edition: _z9781071616529
776	0	8	_iPrinted edition: _z9781071616543
776	0	8	_iPrinted edition: _z9781071616550
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1653-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eIG _zSI