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008			220929s2022 xxu| s |||| 0|eng d
020			_a9781071625699
024	7		_a10.1007/978-1-0716-2569-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aTranslational Research Methods in Neurodevelopmental Disorders _cedited by Stéphane Martin, Frédéric Laumonnier.
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIII, 264 páginas) _b50 ilustraciones, 44 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v185
505	0		_aUsing Lentiviral shRNA Delivery to Knock-Down Proteins in Cultured Neurons and In Vivo -- Synaptosomal Preparation and Its Application in Preclinical Studies -- Assessing the Nanoscale Organization of Excitatory and Inhibitory Synapses using Recombinant Probes to Visualize Endogenous Synaptic Proteins -- Agonist-Induced Functional Analysis and Cell Sorting (aiFACS) to Select and Characterize Interneurons during Brain Development -- A CRISPR/Cas9-Based Toolkit to Test Gain- and Loss-of-Gene Function in Brain Organoids -- Assessment of Adult Mouse Brain NeuroAnatomical Phenotypes using Quantitative and Precision Histology -- Leveraging Microelectrode Array Technology for Phenotyping Stem Cell-Derived Neurodevelopmental Disease Models -- Humanized Chimeric Mouse Models to Study Human Neural Development and Pathogenesis of Brain Diseases -- In Vivo Manipulations to Correct for Behavioral Phenotypes in Cognitive Disorder Mouse Models -- Assessing Dyadic Social Interactions in Rodent Models of Neurodevelopmental Disorders -- Central and Peripheral Explorations by Metabolomics to Study Neurodevelopmental Disorders -- Genetic Engineering of Non-Human Primates Models for Studying Neurodevelopmental Disorders.
520			_aThis volume explores the latest techniques used to study neurodevelopmental diseases (NDD) that range from molecular aspects to integrated research approaches and brain imaging in living rodents. Chapters in this book cover topics such as protocols to deliver shRNA in vitro and in vivo using lentiviral particles to knock-down specific protein expression; experimental procedures to use recombinant fluorescent probes to visualize endogenous proteins at the mammalian synapse; CRISPR/Cas9 toolkit to assess either gain- or loss-of-gene function in brain organoids; detailed protocols to use in vivo manipulations to correct the behavioral phenotypes in cognitive disorder mouse lines; and experimental approaches to genetically engineer macaque models of NDDs and investigate how genetic predisposition may cause neural and functional alterations. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and practical, Translational Research Methods in Neurodevelopmental Disorders is a valuable resource for all researchers who want to learn more about this important and developing field. .
700	1		_aMartin, Stéphane _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aLaumonnier, Frédéric _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071625682
776	0	8	_iPrinted edition: _z9781071625705
776	0	8	_iPrinted edition: _z9781071625712
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2569-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
988			_aSpringer_Protocols_2022
999			_c396427 _d396427