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020			_a9781071625811
024	7		_a10.1007/978-1-0716-2581-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aLipopolysaccharide Transport _bMethods and Protocols _cedited by Paola Sperandeo.
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (X, 292 páginas) _b54 ilustraciones, 39 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2548
505	0		_aUse of Mutagenesis and Functional Screens to Characterize Essential Genes Involved in Lipopolysaccharide Transport -- Generation of Stable and Unmarked Conditional Mutants in Pseudomonas aeruginosa -- Analysing the Function of Essential Genes by Plasmid Shuffling -- Membrane Fractionation by Isopycnic Sucrose Density Gradient Centrifugation for Qualitative Analysis of LPS in Escherichia Coli -- Exploring The Topology of Cytoplasmic Membrane Proteins Involved in Lipopolysaccharide Biosynthesis by in silico and Biochemical Analyses -- Use of Site‐Directed Spin Labeling EPR Spectroscopy to Study Protein-LPS Interactions -- A Photo-Crosslinking Approach to Monitor Assembly of an LptD Intermediate with LptE in a Living Cell -- Mass Spectrometry Analysis of Dynamics and Interactions of the LPS translocon LptDE -- Affinity Purification and Co-Immunoprecipitation of Trans-envelope Protein Complexes in Gram-negative Bacteria -- A Bacterial Two-Hybrid System for in vivo assays of Protein-Protein Interactions and Drug Discovery -- Detecting Lipoproteins Sneaking out of the Lipopolysaccharide Leaflet -- Dissecting Lipopolysaccharide Composition and Structure by GC-MS and MALDI Spectrometry -- Probing the Functional Interaction Interface of Lipopolysaccharide and Antimicrobial Peptides: A Solution-State NMR Perspective -- Cryo-EM Analysis of the Lipopolysaccharide Flippase MsbA -- Protein Crystallization of Two Recombinant Lpt Proteins -- Metabolic Incorporation of Azido-Sugars into LPS to Enable Live Cell Fluorescence Imaging -- Use of Atomic Force Microscopy to Characterize LPS Perturbations.
520			_aThis volume provides the most widely used protocols and techniques employed in lipopolysaccharide( LPS) transport studies. Chapters detail identification and analysis of essential genes and crucial mutations in LPS transport-related genes, LPS profile determination, LPS chemical analysis, protein-LPS interaction, and LPS visualization. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Lipopolysaccharide Transport: Methods and Protocols aims to deliver an overview on the multidisciplinary study of LPS transport and to identify new and innovative antibacterial strategies.
700	1		_aSperandeo, Paola _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071625804
776	0	8	_iPrinted edition: _z9781071625828
776	0	8	_iPrinted edition: _z9781071625835
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2581-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
988			_aSpringer_Protocols_2022
999			_c396426 _d396426