000			03243nam a22003735i 4500
001			396419
003			ES-MaUEC
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007			cr nn 008mamaa
008			210819s2022 xxu| s |||| 0|eng d
020			_a9781071615898
024	7		_a10.1007/978-1-0716-1589-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aImaging and Quantifying Neuronal Autophagy _cedited by Ben Loos, Esther Wong.
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XII, 150 páginas) _b33 ilustraciones, 27 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v171
505	0		_aQuantification of Autophagosome Size and Formation Rate by Electron and Fluorescence Microscopy in Baker's Yeast -- Ultrastructure of the Macroautophagy Pathway in Mammalian Cells -- Live Imaging of Autophagosome Biogenesis and Maturation in Primary Neurons -- Monitoring Autophagic Activity In Vitro and In Vivo using the GFP-LC3-RFP-LC3∆G Probe -- Measuring Autophagic Flux in Neurons by Optical Pulse Labeling -- Measuring Autophagosome Flux -- Measurement of Neuronal Tau Clearance In Vivo -- Methods for Studying Axonal Autophagosome Dynamics in Adult Dorsal Root Ganglion Neurons -- Imaging and Quantifying Neuronal Autophagy to Determine the Autophagy Contribution to Neuronal and Dendritic Morphogenesis -- Correlative Light and Electron Microscopy (CLEM): Bringing Together the Best of Both Worlds to Study Neuronal Autophagy.
520			_aThe volume aims to explore the dynamic nature of the autophagy pathway, and the latest techniques that allow researchers to capture and quantify this process in neurons. The chapters in this volume cover topics such as fundamental, historical, and functional approaches that began in baker's yeast; Saccharomyces cerevisiae; the role of both electron microscopy and live-cell imaging using fluorescently tagged autophagy proteins; and the rate of puncta appearance and its correlation with the rate of autophagosome formation. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and practical, Imaging and Quantifying Neuronal is a valuable resource that provides insights into the power of microscopy tools, live-cell imaging, and photoactivation and correlative techniques. .
700	1		_aLoos, Ben _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWong, Esther _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781071615881
776	0	8	_iPrinted edition: _z9781071615904
776	0	8	_iPrinted edition: _z9781071615911
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-1589-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
988			_aSpringer_Protocols_2022
999			_c396419 _d396419