000 04005nam a2200409 i 4500
999 _c396416
_d396416
001 396416
003 ES-MaUEC
005 20230612145412.0
006 a|||| o|||| 00| 0
007 cr nn 008mamaa
008 220910s2022 xxua o |||| 0|eng d
020 _a9781071625538
024 7 _a10.1007/978-1-0716-2553-8
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH671
_b2022 EB
245 0 0 _aApoptosis and Cancer :
_bMethods and Protocols
_cedited by Hugo Barcenilla, David Diaz
250 _a1st edition 2022
264 1 _aNew York, NY
_bSpringer International Publising
_c2022
300 _a1 recurso en línea (XII, 212 páginas)
_b34 ilustraciones, 32 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v2543
505 0 _aA Non-Destructive, Real-Time Annexin V Apoptosis Assay -- Cell Death Analysis in Cancer Spheroids from a Microfluidic Device -- Apoptosis Detection by Quantification of Cell Debris in Bright-Field Microscopy Images -- Accurate Enumeration of Apoptotic Cancer Cells Using Flow Cytometry -- Assessment of the Immune Response to Tumor Cell Apoptosis and Efferocytosis -- Quantitative Analysis of Apoptosis and Necrosis in Live Cells Using Flow Cytometry -- A Fluorescence-Microscopic System for Monitoring the Turnover of the Autophagic Substrate p62/SQSTM1 -- Single-Cell Profiling of the Intrinsic Apoptotic Pathway by Mass Cytometry (CyTOF) -- High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens using Fluorescent Barcoding -- Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood -- Quantification of Neutrophils Undergoing NET Formation and Distinguishing Mechanisms of Neutrophil Cell Death by Use of a High-Throughput Method -- Profiling of Protein Carbonylations in Ferroptosis by Chemical Proteomics -- Cell Cycle Analysis of ER Stress and Autophagy -- Proximity Ligation In Situ Assay to Monitor Autophagy-Related Protein Interactions and Autophagy in Cancer Cells -- Simultaneous Detection of Inflammasome Activation and Membrane Damage During Pyroptosis -- Cancer and Apoptosis.
520 _aThis volume discusses methods used in the fields of molecular and cellular biology for detecting and studying cell death, especially in cancer and cancer therapy. Chapters in this book cover topics such as non-destructive, real-time Annexin V apoptosis assay; assessment of the immune response to tumor cell apoptosis and efferocytosis; mass cytometry assessment of cell phenotypes and signaling states in human whole blood; cell cycle analysis of ER stress and autophagy; and simultaneous detection of inflammasome activation and membrane damage during pyroptosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Apoptosis and Cancer: Methods and Protocols is a valuable resource and practical guide for both novice and expert researchers looking into the "meaning of death.".
988 _aSpringer_Protocols_2022
650 7 _2embne
_9157983
_aApoptosis
_vManuales de laboratorio
650 7 _2embne
_9146156
_aCélulas cancerosas
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781071625521
776 0 8 _iPrinted edition:
_z9781071625545
776 0 8 _iPrinted edition:
_z9781071625552
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2553-8
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b06/2023
_dz
_eb
_zSI