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008			220909s2022 xxua o |||| 0|eng d
020			_a9781071625453
024	7		_a10.1007/978-1-0716-2545-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH440.5 _b2022 EB
245	0	0	_aDNA-Encoded Chemical Libraries : _bMethods and Protocols _cedited by David Israel, Yun Ding
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (X, 226 páginas) _b76 ilustraciones, 64 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2541
505	0		_aLibrary Synthesis: Building Block Selection, Handling, and Tracking -- Library Synthesis: Building Block Validation -- Library Synthesis and Analytics -- Chemical Ligation of Oligonucleotide Tags to Support Encoded Chemical Library Synthesis -- On-DNA Reductive Amination and Alkylation -- On-DNA-1,2,3-Triazole Formation via Click Reaction -- On-DNA Suzuki-Miyaura Cross-Coupling -- On-DNA Pd and Cu Promoted C-N Cross-Coupling Reactions -- Palladium Promoted On-DNA Heck Reactions -- Ruthenium Promoted On-DNA C-H Activation Reaction -- DNA-Compatible Nitro Reduction and Synthesis of Benzimidazoles -- Condensation of DNA-Conjugated Imines with Homophthalic Anhydride for the Synthesis of Isoquinolones On-DNA -- DNA Encoding of Natural Products -- Initiating DNA-Encoded Library Synthesis with a Hexathymidine DNA Oligonucleotide -- One Bead-One Compound (OBOC) Peptidomimetic Encoded Library Synthesis via Split-and-Pool Methods -- DNA Conjugates as Tool Compounds for DEL Selections -- Quantitation of DNA Encoded Libraries by qPCR -- Target Protein Design and Pre-Selection Analysis -- DEL Selections Against a Soluble Protein Target -- Selection Method of DNA-Encoded Chemical Library for Irreversible Covalent Binders -- Next Generation Sequencing of DNA-Encoded Libraries -- Translation of DNA Sequence to Chemical Structure in DNA Encoded Libraries -- Analysis of DNA-Encoded Library Screening Data: Selection of Molecules for Synthesis -- High Throughput Binder Confirmation (HTBC) -- High-Throughput Binder Confirmation Using Affinity Selection Mass Spectrometry.
520			_aThis volume discusses protocols that cover synthesis, screening by selection, and analysis of DNA-encoded chemical libraries (DEL). Chapters in this book focus on methods used to practice DEL technology and include solution phase library synthesis using a variety of chemistries; DNA encoding of chemical structure; design, preparation and analysis of target proteins and tool compounds; screening of soluble protein targets by affinity selection; DEL qPCR, preparative PCR and DNA sequence analysis; computational methods used to analyze selections and choose compounds for resynthesis; and analysis of hit compounds. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, DNA-Encoded Chemical Libraries: Methods and Protocols is a valuable resource for scientists interested in DEL technology for drug discovery, and will contribute to the continued advancement in this important field.
988			_aSpringer_Protocols_2022
650		7	_2embne _9138044 _aGenética _vManuales de laboratorio
650		7	_2embne _9141808 _aQuímica farmacéutica _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071625446
776	0	8	_iPrinted edition: _z9781071625460
776	0	8	_iPrinted edition: _z9781071625477
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2545-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eb _zSI