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008			220831s2022 xxua o |||| 0|eng d
020			_a9781071627365
024	7		_a10.1007/978-1-0716-2736-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH605 _b2022 EB
245	0	0	_aCell-Cycle Synchronization : _bMethods and Protocols _cedited by Zhixiang Wang
250			_a1st edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XI, 250 páginas) _b48 ilustraciones, 34 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2579
505	0		_aCell Cycle Progression and Synchronization: An Overview -- The Trypanosomatids Cell Cycle: A Brief Report -- Cell Cycle-Related Clinical Applications -- Flow Cytometry and Cell Cycle Analysis: An Overview -- Synchronization of Cultured Cells to G1, S, G2, and M Phases by Double Thymidine Block -- Cell Synchronization Techniques for Studying Mitosis -- Synchronization of HeLa Cells to Various Interphases Including G1, S, and G2 Phases -- Synchronization of HeLa Cells to Mitotic Subphases -- Cell Cycle Synchronization of Primary and Cultured Articular Chondrocytes -- Synchronization of Leishmania amazonensis Cell Cycle using Hydroxyurea -- Synchronization of Trypanosoma brucei by Counter-Flow Centrifugal Elutriation -- Synchronization of Saccharomyces cerevisiae Cells for Analysis of Progression through the Cell Cycle -- Cell Cycle Synchrony Methods for Fission Yeast, Schizosaccharomyces pombe -- Analysis of Cell Cycle by Flow Cytometry -- Analysis of Cell Proliferation by Three-Dimensional Culture -- BrdU Incorporation Assay to Analyze the Entry into S Phase -- Assessment of Growth Plate Chondrocytes Proliferative Activity in Embryonic Endochondral Ossification via Ki67 Immunofluorescence -- Detecting Cell Cycle Stage and Progression in Fission Yeast, Schizosaccharomyces pombe.
520			_aThis volume covers a broad range of cell types including cultured cell lines, primary cells, and various unicellular organisms such as fission yeast, budding yeast, parasite Leishmania amazonensis, and parasite Trypanosoma brucei. The chapters in this book are organized into four parts. Part One looks at a general overview of cell cycle control and synchronization. Part Two discusses techniques to synchronize mammalian cells to various cell cycle phases including mitotic sub-phases. Part Three covers synchronization of unicellular organisms and Part Four analyzes cell cycle progression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cell-Cycle Synchronization: Methods and Protocols is a valuable resource for both novice and expert scientists in this developing field.
988			_aSpringer_Protocols_2022
650		7	_2embne _9689279 _aDivisión celular _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071627358
776	0	8	_iPrinted edition: _z9781071627372
776	0	8	_iPrinted edition: _z9781071627389
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2736-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eb _zSI