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008			220830s2022 xxua o |||| 0|eng d
020			_a9781071627075
024	7		_a10.1007/978-1-0716-2707-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC669 _b2022 EB
245	0	0	_aCardiac Gene Therapy : _bMethods and Protocols _cedited by Kiyotake Ishikawa
250			_a2nd edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIV, 347 páginas) _b78 ilustraciones, 69 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2573
505	0		_aUpdates on Cardiac Gene Therapy Research and Methods: Overview of Cardiac Gene Therapy -- Tough Decoy-Mediated Cardiac Gene Suppression -- Direct Reprogramming of Adult Human Cardiac Fibroblasts into Induced Cardiomyocytes using miRcombo -- CRISPR/Cas9 Gene Editing of RYR2 in Human iPSC Derived Cardiomyocytes to Probe Ca2+ Signaling Aberrancies of CPVT Arrhythmogenesis -- Enhancing Cardiomyocyte Transcription using In Vivo CRISPR/Cas9 Systems -- AAV Mediated Somatic Gene Editing for Cardiac and Skeletal Muscle in a Large Animal Model -- Optimization of Synthesis of Modified mRNA -- Design and Production of Heart Chamber-Specific AAV9 Vectors -- Generation of Atrial-Specific Construct using Sarcolipin Promoter Associated CRM4 Enhancer -- Cardiac Targeted Adeno-Associated Virus Injection in Rats -- Cardiac Gene Delivery in Large Animal Models-Antegrade Techniques -- Locked Nucleic Acid AntimiR Therapy for the Heart -- Cardiac Gene Delivery in Large Animal Models: Selective Retrograde Venous Injection -- Endocardial Gene Delivery using NOGA Catheter System -- Surgical Methods for Cardiac Gene Delivery in Large Animals -- Atrial Gene Painting in Large Animal Model of Atrial Fibrillation -- Stent-Based Gene Delivery for Coronary Disease -- Selective Anti-AAV Antibody Depletion by Hemapheresis and Immunoadsorption -- Ex Vivo Delivery of Viral Vectors by Organ Perfusion for Cardiac Transplantation Gene Therapy -- Intra-Airway Gene Delivery for Pulmonary Hypertension in Rodent Models -- Endo-Bronchial Gene Delivery for Pulmonary Hypertension in a Large Animal Model -- Cell-Based Determination of Neutralizing Antibodies against Adeno-Associated Virus in Cardiac Gene Therapy -- Left Ventricular Pressure Volume Assessment Using Carotid Artery Access in the Rat -- Assessing the Effect of Cardiac Gene Therapy using Catheter-Based Pressure-Volume Measurement in Large Animals -- ELIspot Assay for Gene Therapy in Large Animal Studies -- Assessing Recombinant AAV Shedding after Cardiac Gene Therapy.
520			_aThis second edition volume expands on the previous edition with updated techniques and discussions on topics such as gene suppression, editing, and reprogramming; cardiac gene therapy vectors and promoters; cardiac gene delivery methods; pulmonary hypertension; and patient screening and measuring the efficacy of cardiac gene therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Cardiac Gene Therapy: Methods and Protocols, Second Edition is a valuable tool for researchers in cardiology who are conducting gene therapy research. This book will lead to further advancements and successful clinical translations in the field. Chapter "Direct Reprogramming of Adult Human Cardiac Fibroblasts into Induced Cardiomyocytes Using miRcombo" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
988			_aSpringer_Protocols_2022
650		7	_2embne _9169062 _aAparato circulatorio _xEnfermedades _xAspectos genéticos _vManuales de laboratorio
650		7	_2embne _9144879 _aGenética médica _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071627068
776	0	8	_iPrinted edition: _z9781071627082
776	0	8	_iPrinted edition: _z9781071627099
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2707-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eb _zSI