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008			220825s2022 xxua o |||| 0|eng d
020			_a9781071625491
024	7		_a10.1007/978-1-0716-2549-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR245 _b2022 EB
245	0	0	_aCandida Species : _bMethods and Protocols _cedited by Richard Calderone
250			_a2nd edition 2022
264		1	_aNew York, NY _bSpringer International Publising _c2022
300			_a1 recurso en línea (XIV, 390 páginas) _b63 ilustraciones, 49 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v2542
505	0		_aCRISPR-Mediated Genome Editing in the Human Fungal Pathogen C. albicans -- Plasmid-Based CRISPR-Cas9 Editing in Multiple Candida Species -- A Proteomic Approach for the Quantification of Post-Translational Protein Lysine Acetylation in Candida albicans -- How to Use the Candida Genome Database -- Sub-Telomeric Chromatin Structure by Chromosome Conformation Capture (3C)-qPCR Methodology in Candida glabrata -- Construction of Double Heterozygous Deletion Strains for Complex Haploinsufficiency-Based Genetic Analysis in Candida albicans -- Using Synthetic Genetic Interactions in Candida glabrata as a Novel Method to Detect Genes with Roles in Antifungal Drug Resistance -- Identifying Specific Small Molecule-Protein Interactions Using Target Abundance-Based Fitness Screening (TAFiS) -- High Throughput Phospholipidomics of Candida Cells: From Sample Preparation to Data Analysis -- Genetic Screens of an Anti-Candida Natural Product Using the Heterozygous Saccharomyces cerevisiae Mutant Library -- Assessing Complex I (CI) Mitochondrial Subunit Protein Functions in Candida albicans -- In Vitro Biophysical Characterization of Candidalysin: A Fungal Peptide Toxin -- Assessment of cAMP-PKA Signaling in Candida glabrata by FRET-Based Biosensors -- Methods Related to the Immunopathogenesis of Vulvovaginal Candidiasis and Associated Neutrophil Anergy -- A Simple Method for Growth of Candida albicans Biofilms under Continuous Media Flow and for Recovery of Biofilm Dispersed Cells -- A Simple 96 Well Plate-Based Method for Development of Candida Biofilms under Static Conditions -- Comparative Analysis of the Fitness of Candida albicans Strains during Colonization of the Mice Gastrointestinal Tract -- Detection and Identification of Candida auris from Clinical Skin Swabs -- Production and Isolation of the Candida Species Biofilm Extracellular Matrix -- Characterization of the Effects of Candida Gastrointestinal Colonization on Clostridioides difficile Infection in a Murine Model -- Analysis of Gut Microbiome Using Fecal Samples -- Measurements of Treg Cell Induction by Candida albicans DNA Using Flow Cytometry -- Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure -- Isolation, Physicochemical Characterization, Labeling, and Biological Evaluation of Mannans and Glucans -- Understanding the Protective Role of IL-17 during Oropharyngeal Candidiasis.
520			_aThis updated volume explores Candida and candidiasis methods, useful to a wide variety of Candida scientists including those new to the field. Beginning with a section on the Candida genome, the book continues by covering techniques for antifungal discovery and studying resistance, pathogenesis and virulence, communities, as well as immunity. Biofilm protocols are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Candida Species: Methods and Protocols, Second Edition serves as an ideal guide for researchers working to further our understanding of this family of infective fungi.
988			_aSpringer_Protocols_2022
650		7	_2embne _9672194 _aHongos patógenos _vManuales de laboratorio
650		7	_2embne _9140738 _aGenética microbiana _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781071625484
776	0	8	_iPrinted edition: _z9781071625507
776	0	8	_iPrinted edition: _z9781071625514
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-0716-2549-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b06/2023 _dz _eb _zSI