000 04351nam a22003975i 4500
999 _c393747
_d393747
_x1
001 393747
003 ES-MaUEC
005 20240314174426.0
006 a||||fo|||| 00| 0
007 cr nn 008mamaa
008 110418s2009 xxu| o |||| 0|eng d
020 _a9781597452410
024 7 _a10.1007/978-1-59745-241-0
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP106.6
_b2009 EB
245 0 0 _aAngiogenesis Protocols
_cedited by Stewart Martin, Cliff Murray
250 _a2nd edition 2009
264 1 _aTotowa, NJ
_bHumana Press
_c2009
300 _a1 recurso en línea (XI, 358 páginas)
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v467
505 0 _aReview Articles -- Angiogenic Signalling Pathways -- Angiogenic Signalling Pathways -- Microscopic Assessment -- Assessing Tumor Angiogenesis in Histological Samples -- Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis -- Cell Isolation Techniques -- Vascular Endothelial Cells from Human Micro- and Macro-vessels: Isolation, Characterisation and Culture -- Lymphatic Endothelial Cells: Establishment of Primaries and Characterization of Established Lines -- Isolation, Culture and Characterisation of Vascular Smooth Muscle Cells -- Circulating Endothelial Progenitor Cells (CEPC) -- In vitro Techniques -- In vitro Assays for Endothelial Cell Functions Related to Angiogenesis: Proliferation, Motility, Tubular Differentiation and Proteolysis -- Tube Formation: an In Vitro Matrigel Angiogenesis Assay -- Three-dimensional In vitro Angiogenesis in the Rat Aortic Ring Model -- Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature -- Genetic Manipulation of Corneal Endothelial Cells: Transfection and Viral Transduction -- Creation of Human Skin Equivalents for the In vitro Study of Angiogenesis in Wound Healing -- In vivo Techniques -- Measurement of Angiogenic Phenotype by Use of a Two Dimensional Mesenteric Angiogenesis Assay -- Quantitative Estimation of Tissue Blood Flow Rate -- In Vivo Matrigel Migration and Angiogenesis Assay -- The Sponge Implant Model of Angiogenesis -- The Dorsal Skinfold Chamber: Studying Angiogenesis by Intravital Microscopy -- The Corneal Pocket Assay -- Use of the Hollow Fibre Assay for Studies on Tumor Neovasculature -- The Cranial Bone Window Model: Studying Angiogenesis of Primary and Secondary Bone Tumors by Intravital Microscopy.
520 _aAs experimentation and clinical trials with first generation anti-angiogenic agents have yielded results and our understanding of the biology and physiology of blood and lymphatic vessels has increased, a new angiogenesis volume swiftly became a necessity. Angiogenesis Protocols, Second Edition remains true to its original vision of providing a single source for angiogenesis researchers, irrespective of levels of resource and expertise, by collecting a range of methods for cell isolation and assessing angiogenesis in vivo or in vitro. This information, however, is expanded to include chapters on circulating endothelial progenitor cells, angiogenic signalling pathways, imaging of angiogenesis, and measurement of tissue blood flow. Written in the Methods in Molecular Biology™ series format, the chapters provide step-by-step laboratory protocols, lists of necessary materials and reagents, and a Notes section, which details tips on troubleshooting and avoiding known pitfalls. Extensive and cutting-edge, Angiogenesis Protocols, Second Edition is not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis.
988 _aSpringer_Protocols_2009
650 7 _2embne
_9164656
_aNeovascularización
776 0 8 _iPrinted edition:
_z9781607610700
776 0 8 _iPrinted edition:
_z9781588299079
776 0 8 _iPrinted edition:
_z9781627038010
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-241-0
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b01/2024
_dz
_ek
_zSI