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020			_a9781592591152
024	7		_a10.1385/1592591159 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP572 .S7 _b2001 EB
245	0	0	_aSteroid Receptor Methods : _bProtocols and Assays _cedited by Benjamin A. Lieberman
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (XIV, 381 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v176
505	0		_aBioinformatics -- Bioinformatics of Nuclear Receptors -- Phylogenetic Inference and Parsimony Analysis -- Purification Protocols -- Expression and Purification of Recombinant Human Progesterone Receptor in Baculovirus and Bacterial Systems -- High-Yield Purification of Functionally Active Glucocorticoid Receptor -- Production and Purification of Histidine-Tagged Dihydrotestosterone-Bound Full-Length Human Androgen Receptor -- Large Scale Production of Nuclear Receptor Ligand-Binding Domains -- Physical Structure of Nuclear Receptor-DNA Complexes -- Isolation of Steroid-Regulated Genes from the Uterus by mRNA Differential Display -- Identification of Nuclear Hormone Receptor Homologs by Screening Libraries with Highly Degenerate Oligonucleotide Probes -- Steroid Hormone-Binding Assays -- Use of [99mTc]Technetium-Labeled Steroids as Probes for Steroid Hormone Receptors -- Steroid Hormone Metabolites and Hormone Binding Assays -- Vitamin D3 Analog Screening -- Protein Interaction Assays -- Application of Green Fluorescent Protein to the Study of Dynamic Protein-Protein Interactions and Subcellular Trafficking of Steroid Receptors -- Knockout Mice and Steroid Receptor Research -- Yeast Two-Hybrid Screening for Proteins that Interact with Nuclear Hormone Receptors -- Isolation of a p300/CBP Cointegrator-Associated Protein Coactivator Complex -- Nonradioactive Photoaffinity Labeling of Steroid Receptors Using Western Blot Detection System -- Analysis of Steroid Hormone-Induced Histone Acetylation by Chromatin Immunoprecipitation Assay -- Analyzing the Contributions of Chromatin Structure in Nuclear Hormone Receptor Activated Transcription In Vivo -- Cotransfection Assays and Steroid Receptor Biology -- Estrogen Receptor mRNA In Situ Hybridization Using Microprobe System -- Cancer Research and Drug Discovery -- Solid Tumor Cancer Markers and Applications to Steroid Hormone Research -- Assessing Modulation of Estrogenic Activity of Environmental and Pharmaceutical Compounds Using MCF-7 Focus Assay -- Combinatorial Chemistry in Steroid Receptor Drug Discovery -- Identification of Nuclear Receptor Interacting Proteins Using Yeast Two- Hybrid Technology.
520			_aResearch into steroid receptor biology holds great promise for the treatment of cancer and endocrine disorders and for elucidating the fundamental biological mechanisms of gene expression. In Steroid Receptor Methods: Protocols and Assays, a distinguished team of principal investigators and their associates describe in step-by-step detail a cross-section of the latest research techniques available for studying the endocrine system. As a basis for sophisticated biochemical analysis of receptor properties, the contributors provide methods for the production and purification of a variety of receptors, including progesterone, glucocorticoid, and androgen. Other protocols allow the reader to experiment with DNA-binding characteristics and hormone-binding assays and with the use of combinatorial chemistry for drug discovery. A series of novel methods utilizing the latest advances in immunochemistry, yeast two-hybrid screening, and fluorescence are included for the detection and analysis of a variety of cellular proteins that influence steroid receptor effectiveness. Finally, a discussion by example presents the most current bioinformatics tools and analytical techniques. Timely and highly accessible, Steroid Receptor Methods: Protocols and Assays provides biochemists and molecular biologists studying endocrine systems with a full range of the powerful tools needed for elucidating protein interactions with receptors and DNA, as well as for drug discovery.
988			_aSpringer_Protocols_2001
650		7	_2embne _9684920 _aEsteroides
650		7	_2embne _9138639 _aBioquímica
776	0	8	_iPrinted edition: _z9781617371707
776	0	8	_iPrinted edition: _z9780896037540
776	0	8	_iPrinted edition: _z9781489942043
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591159 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _ek _zSI