000			04333nam a22003975i 4500
999			_c393740 _d393740 _x1
001			393740
003			ES-MaUEC
005			20240314174424.0
006			a||||fo|||| 00| 0
007			cr nn 008mamaa
008			100301s2007 xxu| o |||| 0|eng d
020			_a9781597450300
024	7		_a10.1007/978-1-59745-030-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP113.2 _b2007 EB
245	0	0	_aCardiac Gene Expression : _bMethods and Protocols _cedited by Jun Zhang, Gregg Rokosh
250			_a1st edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XII, 366 páginas) _b66 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v366
505	0		_aCardiac Gene Expression -- Microarray Analysis of Gene Expression in Murine Cardiac Graft Infiltrating Cells -- Expression Profiling Using Affymetrix GeneChip® Probe Arrays -- Serial Analysis of Gene Expression (SAGE) -- Functional Genomics by cDNA Subtractive Hybridization -- Statistical Methods in Cardiac Gene Expression Profiling -- Cardiac Gene Regulation -- Measurement of Cardiac Gene Expression by Reverse Transcription Polymerase Chain Reaction (RT-PCR) -- Quantitative (Real-Time) RT-PCR in Cardiovascular Research -- RNase Protection Assay for Quantifying Gene Expression Levels -- In Situ Hybridization -- Cardiac Gene Regulation -- Characterization of cis-Regulatory Elements and Transcription Factor Binding -- Mapping Transcriptional Start Sites and In Silico DNA Footprinting -- Characterization of Cardiac Gene Promoter Activity -- In Silico Assessment of Regulatory cis-Elements and Gene Regulation -- Comparative Genomics -- Developing Computational Resources in Cardiac Gene Expression -- Cardiac Single Nucleotide Polymorphisms -- In Silico Analysis of SNPs and Other High-Throughput Data -- Discovery and Identification of Sequence Polymorphisms and Mutations with MALDI-TOF MS -- Gene Overexpression and Targeting in the Myocardium -- Conditional Targeting -- Cardiomyocyte Preparation, Culture, and Gene Transfer -- Adeno-Associated Viral Vector-Delivered Hypoxia-Inducible Gene Expression in Ischemic Hearts -- Lentivirus-Mediated Gene Expression.
520			_aCardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function. Section I, Cardiac Gene Expression Profiling: The Global Perspective, discusses several different approaches to examining, identifying, and analyzing changes in transcriptome gene expression. Section II, Cardiac Gene Regulation: Gene-Specific mRNA Measurement in the Myocardium, outlines more sensitive and gene-targeted expression methods. Section III, Cardiac Gene Regulation: Promoter Characterization in the Myocardium, provides protocols for the study of underlying gene regulation mechanisms by focusing on the interaction of transcription factors with their cognate cis binding elements. Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome and other model genomes. The concluding section, Gene Overexpression and Targeting in the Myocardium, highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion.
988			_aSpringer_Protocols_2007
650		7	_2embne _9686405 _aMiocardio _xRegeneración
650		7	_2embne _9162770 _aExpresión génica
776	0	8	_iPrinted edition: _z9781617375149
776	0	8	_iPrinted edition: _z9781588293527
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-030-0 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _ek _zSI