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020 _a9781493918751
024 7 _a10.1007/978-1-4939-1875-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQH603 .M5
_b2015 EB
245 0 0 _aMitochondrial Regulation :
_bMethods and Protocols
_cedited by Carlos M. Palmeira, Anabela P. Rolo
250 _a1st edition 2015
264 1 _aNew York, NY
_bSpringer International Publishing
_c2015
300 _a1 recurso en línea (XI, 201 páginas)
_b38 ilustraciones, 26 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1241
505 0 _aIsolation of Crud Mitochondrial Fraction from Cells -- Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein - Analyses of MicroRNA and Protein Expressions -- PCR Based Determination of Mitochondrial DNA Copy Number in Multiple Species -- NAD+ Content and its Role in Mitochondria -- Measuring PGC-1α and its Acetylation Status in Mouse Primary Myotubes -- Measurement of Mitochondrial Oxygen Consumption Rates in Mouse Primary Neurons and Astrocytes -- Determination Of Oxidative Phosphorylation Complexes Activities -- Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Muscle Biopsies from Patients with Mitochondrial Defects -- A Method Aimed at Assessing the Functional Consequences of the Supramolecular Organization of the Respiratory Electron Transfer Chain by Time-resolved Studies -- BN-PAGE-based Approach to Study Thyroid Hormones and Mitochondrial Function -- Detection of UCP1 Protein and Measurements of Dependent GDP-Sensitive Proton Leak in Non-Phosphorylating Thymus Mitochondria -- Exploring Liver Mitochondrial Function By 13c-Stable Isotope Breath Tests. Implications In Clinical Biochemistry -- Following Mitochondria Dynamism: Confocal Analysis of The Organelle Morphology -- Analysis of Pro-apoptotic Protein Trafficking to and From Mitochondria -- Mitophagy and Mitochondrial Balance.
520 _aIn this volume expert researchers in the field detail the many methods for further research into regulation of mitochondrial function.  Chapters focus on mitochondria with other cellular components, discussing how these interactions influence the dynamics of mitochondrial structure and biogenesis. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Mitochondrial Regulation: Methods and Protocols seeks to aid advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry and bioenergetics.
988 _aSpringer_Protocols_2015
650 7 _2embne
_9147980
_aMitocondrias
650 7 _2embne
_9165668
_aFisiología celular
776 0 8 _iPrinted edition:
_z9781493918744
776 0 8 _iPrinted edition:
_z9781493918768
776 0 8 _iPrinted edition:
_z9781493946129
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1875-1
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b01/2024
_dz
_ek
_zSI