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008			100301s2006 xxu| o |||| 0|eng d
020			_a9781597450263
024	7		_a10.1385/159745026X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2006 EB
245	0	0	_aNew and Emerging Proteomic Techniques _cedited by Dobrin Nedelkov, Randall W. Nelson
250			_a1st edition 2006
264		1	_aTotowa, NJ _bHumana Press _c2006
300			_a1 recurso en línea (248 páginas) _b67 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v328
505	0		_aOn-Chip Protein Synthesis for Making Microarrays -- RCA-Enhanced Protein Detection Arrays -- Antibody Microarrays Using Resonance Light-Scattering Particles for Detection -- Chemical Proteomics Profiling of Proteasome Activity -- Two-Dimensional Difference Gel Electrophoresis -- Oligomeric States of Proteins Determined by Size-Exclusion Chromatography Coupled With Light Scattering, Absorbance, and Refractive Index Detectors -- Surface Plasmon Resonance Imaging Measurements of Protein Interactions With Biopolymer Microarrays -- Surface Plasmon Resonance Mass Spectrometry for Protein Analysis -- High-Throughput Affinity Mass Spectrometry -- Isotope-Coded Affinity Tags for Protein Quantification -- Proteomic Analysis by Multidimensional Protein Identification Technology -- Isolation of Glycoproteins and Identification of Their N-Linked Glycosylation Sites -- N-Glycosylation Analysis Using the StrOligo Algorithm -- MALDI-MS Data Analysis for Disease Biomarker Discovery -- Using the Global Proteome Machine for Protein Identification.
520			_aNumerous emerging technologies have propelled the growth of proteomics into a wide-ranging discipline with great potential to improve human health through the discovery of new protein biomarkers and therapeutics. In New and Emerging Proteomic Techniques, a panel of leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods described here span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. Where necessary, two or more distinct techniques are provided for a given problem, and advanced bioinformatics approaches are presented for the analysis of complex proteomic data. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, New and Emerging Proteomic Techniques offers expert and novice investigators alike today's most promising proteomics techniques for probing the biological processes and understanding the diseases of the human body.
988			_aSpringer_Protocols_2006
650		7	_2embne _9430647 _aProteómica
776	0	8	_iPrinted edition: _z9781617376153
776	0	8	_iPrinted edition: _z9781588295194
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159745026X _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _ek _zSI