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008			100301s2006 xxu| o |||| 0|eng d
020			_a9781597450539
024	7		_a10.1007/978-1-59745-053-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050			_aTP248.65 .I45 _b2006 EB
245	0	0	_aImmobilization of Enzymes and Cells _cedited by José M. Guisán
250			_a2nd edition 2006
264		1	_aTotowa, NJ _bHumana Press _c2006
300			_a1 recurso en línea (XIV, 450 páginas) _b217 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Biotechnology _x1940-607X _v22
505	0		_aImmobilization of Enzymes as the 21st Century Begins -- Immobilization of Enzymes -- Cross-Linked Enzyme Aggregates -- Immobilization-Stabilization of Enzymes by Multipoint Covalent Attachment on Supports Activated With Epoxy Groups -- Glutaraldehyde in Protein Immobilization -- Practical Protocols for Lipase Immobilization Via Sol-Gel Techniques -- Encapsulation of Enzymes Using Polymers and Sol-Gel Techniques -- Design of Smart Biocatalysts -- Affinity Immobilization of Tagged Enzymes -- Bioaffinity Immobilization -- One-Step Purification, Immobilization, and Stabilization of Poly-Histidine-Tagged Enzymes Using Metal Chelate-Epoxy Supports -- Stabilization of Multimeric Enzymes Via Immobilization and Further Cross-Linking With Aldehyde-Dextran -- Purification, Immobilization, Hyperactivation, and Stabilization of Lipases by Selective Adsorption on Hydrophobic Supports -- Immobilization and Stabilization of Proteins by Multipoint Covalent Attachment on Novel Amino-Epoxy-Sepabeads® -- Improved Stabilization of Chemically Aminated Enzymes Via Multipoint Covalent Attachment on Glyoxyl Supports -- Stabilization of New Imprint Property of Glucose Oxidase in Pure Aqueous Medium by Cross-Linked-Imprinting Approach -- Reversible Covalent Immobilization of Enzymes Via Their Thiol Groups -- Very Strong But Reversible Immobilization of Enzymes on Supports Coated With Ionic Polymers -- Immobilization of Enzymes on Magnetic Particles -- Immobilization of Proteins on Gold Surfaces -- Immobilization of Enzymes on Electrodes -- Immobilization of Enzymes for Use in Organic Media -- Immobilization of Enzymes for Use in Ionic Liquids -- Immobilization of Enzymes for Use in Supercritical Fluids -- Immobilized Enzymes for Biomedical Applications -- Characterization of Immobilized Enzymes by Microcalorimetry -- Use of Immobilized Biocatalysts in Fluidized Bed Format -- Taylor-Couette Vortex Flow in Enzymatic Reactors -- A Novel Immobilization Method for Entrapment: LentiKats® -- Encapsulation of Cells in Alginate Gels -- Immobilization of Cells on Polyurethane Foam -- Immobilization of Cells With Transition Metal -- Immobilization of Microalgae -- Bioluminescence in Immobilized Cells for Biomass Detection and Biosensor Applications -- A Proteomic Approach to Biofilm Cell Physiology -- Encapsulation of Bacteria for Biodegradation of Gasoline Hydrocarbons -- Biomedical Applications of Immobilized Cells.
520			_aThe continuing rapid progress in work designed to improve the functional properties of enzymes and cells as industrial catalysts has led to this revised, updated, and expanded new edition of the warmly received initial edition of Immobilization of Enzymes and Cells. This long-awaited second edition contains new and simplified protocols useful for industrial applications, novel techniques that will prove useful now or in the near future, and protocols for the preparation of immobilized derivatives suitable for a wide variety of nonconventional reaction media. The authors also offer tools for the development of new immobilization techniques, methods for preparing immobilized derivatives for therapeutic and industrial use, and new chemical reactors designed to overcome the limitations of immobilized derivatives. The emphasis is on improving enzyme and cell properties via very simple immobilization protocols, along with the development of new and better methods. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, Immobilization of Enzymes and Cells, Second Edition, affords biochemists, biotechnologists, and biochemical engineers a practical review of all the latest methods and tools-as well as optimized conventional techniques-needed to carry out successful research involving immobilizing enzymes and cells.
988			_aSpringer_Protocols_2006
650		7	_2embne _9413398 _aBiotecnología _xAplicaciones industriales
650		7	_2embne _9138640 _aEnzimas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617374739
776	0	8	_iPrinted edition: _z9781588292902
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-053-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eu _zSI