000			04677nam a2200397 i 4500
999			_c393702 _d393702
001			393702
003			ES-MaUEC
005			20231227184945.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			100301s2001 xxu| o |||| 0|eng d
020			_a9781592590469
024	7		_a10.1385/1592590462 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP601.7 _b2001 EB
245	0	0	_aMatrix Metalloproteinase Protocols _cedited by Ian M. Clark
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (XV, 545 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v151
505	0		_aMMPs and TIMPs-An Overview of the Field -- MMPs and TIMPs -- Strategies for Cloning New MMPs and TIMPs -- Structural Studies on MMPs andTIMPs Wolfram Bode and Klaus Maskos -- Matrix MetaIIoproteinase Substrate Binding Domains, Modules and Exosites -- The Matrix Metalloproteinase (MMP) and Tissue Inhibitor of Metalloproteinase (TIMP) Genes -- Models for Gain-of-Function and Loss-of-Function of MMPs -- Expression and Purification of MMPs and TIMPs -- Expression of MMPs andTIMPs in Mammalian Cells -- Expression of Recombinant Matrix Metalloproteinases in Escherichia coli -- Expression of Human Collagenase I (MMP-1) andTIMP-1 in a Baculovirus-Based Expression System -- Expression of Recombinant Matrix Metalloproteinases in Yeast -- Expression of Recombinant Membrane-Type MMPs -- Refolding of TIMP-2 from Escherichia coli Inclusion Bodies -- Expression and Refolding of Full-Length HumanTIMP-1 -- Purification of MMPs and TIMPs -- Detection of MMPs and TIMPs -- Monitoring MMP andTIMP mRNA Expression by RT-PCR -- Measuring Transcription of Metalloproteinase Genes -- In Situ Hybridization for Metalloproteinases and Their Inhibitors -- Use of EIA to Measure MMPs and TIMPs -- Immunohistochemistry of MMPs and TIMPs -- Detecting Polymorphisms in MMP Genes -- Assay of MMP and TIMP Activities -- Methods for Studying Activation of Matrix Metalloproteinases -- Assay of Matrix Metalloproteinases Against Matrix Substrates -- Zymography and Reverse Zymography for Detecting MMPs, andTIMPs -- In Situ Zymography -- Detection of Focal Proteolysis Using Texas-Red-Gelatin -- Antibodies to MMP-Cleaved Aggrecan -- Cartilage Proteoglycan Release Assay -- Immunoassay for Collagenase-Mediated Cleavage of Types I and II Collagens -- Collagen Degradation Assays -- Invasion Assays and Matrix Metalloproteinases -- Using Fluorogenic Peptide Substrates to Assay Matrix Metalloproteinases -- Kinetic Analysis of the Inhibition of Matrix Metalloproteinases by Tissue Inhibitor of Metalloproteinases (TIMP) -- Assaying Growth Factor Activity of Tissue Inhibitors of Metalloproteinases.
520			_aFrom the simple discovery in 1962 that resorbing tadpole tail expressed an enzyme (MMP) that could degrade collagen gels, matrix metalloproteinase (MMP) research has advanced to discover more than twenty distinct vertebrate MMPs and four specific inhibitors (TIMPS), a veritable family of enzymes involved in many physiological and pathological processes. In Matrix Metalloproteinase Protocols, leading experts detail proven laboratory techniques for the study of MMPs. The methods include those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a wide variety of circumstances. Each method includes step-by-step instructions and notes on variant applications and pitfalls to avoid. A selective overview of the MMP arena spells out where the field has been, where it is, and where it is going. Comprehensive and highly practical, Matrix Metalloproteinase Protocols brings together the long and hard-earned experience of master experimentalists that will allow not only novices to get up to speed quickly, but also add to the repertoire of successful techniques in expert laboratories.
988			_aSpringer_Protocols_2001
650		7	_2embne _9666050 _aMetaloenzimas
776	0	8	_iPrinted edition: _z9781617371578
776	0	8	_iPrinted edition: _z9781489942142
776	0	8	_iPrinted edition: _z9780896037335
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590462 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI