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020 _a9783662529591
024 7 _a10.1007/978-3-662-52959-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aRB43.7
_b2017 EB
245 0 0 _aFluorescence In Situ Hybridization (FISH) :
_bApplication Guide
_cedited by Thomas Liehr
250 _a2nd edition 2017
264 1 _aBerlin, Heidelberg
_bSpringer International Publising
_c2017
300 _a1 recurso en línea (XIII, 606 páginas)
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aSpringer Protocols Handbooks
_x1949-2456
505 0 _aPart I Required Equipment and Probes for FISH Procedures -- Part II FISH Procedure -- Part III Material Suited for FISH Applications in Humans -- Part IV Multicolor-FISH-Probe Sets (mFISH) and Immunostaining -- Part V Interphase FISH -- Part VI Applications of FISH in Zoology, Botany and Microbiology -- Part VII Comparative Genomic Hybridization (CGH) and Array CGH.
520 _aThis manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear architectures and epigenetic changes, as well as comparative genomic hybridization studies, this 2nd edition describes how FISH can be combined with other techniques. The latter include immunostaining, electron microscopy, single cell electrophoresis and microdissection. This well-received application guide provides essential protocols for beginning FISHers and FISH experts alike working in the fields of human genetics, microbiology, animal and plant sciences.
988 _aSpringer_Protocols_2017
650 7 _2embne
_9158916
_aDiagnóstico molecular
776 0 8 _iPrinted edition:
_z9783662529577
776 0 8 _iPrinted edition:
_z9783662529584
776 0 8 _iPrinted edition:
_z9783662571002
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-3-662-52959-1
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b01/2024
_dq
_eb
_zSI