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020 _a9781627032964
024 7 _a10.1007/978-1-62703-296-4
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP519.9 .C36
_b2013 EB
245 0 0 _aCapillary Electrophoresis of Biomolecules :
_bMethods and Protocols
_cedited by Nicola Volpi, Francesca Maccari
250 _a1st edition 2013
264 1 _aTotowa, NJ
_bHumana Press
_c2013
300 _a1 recurso en línea (XIV, 358 páginas)
_b108 ilustraciones, 30 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
505 0 _aRecent Advances in Capillary Electrophoresis-Based Proteomic Techniques for Biomarker Discovery -- Capillary Electrophoresis-mass Spectrometry of Carbohydrates -- Approaches to Enhancing the Sensitivyty of Carbohydrate Separations in Capillary Electrophoresis -- Determination of Monosaccharides Derivatized with 2-Aminobenzoic Acid by Capillary Electrophoresis -- Determination of Mono- Di- and Oligosaccharides by Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection -- Separation of Chitooligosaccharides in Acidic Solution by Capillary Electrophoresis -- Capillary Electrophoresis for the Analysis of Glycosaminoglycan-derived Disaccharides -- High Throughput Capillary Electrophoresis Mass Spectrometry: From Analysis of Amino Acids to Analysis of Protein Complexes -- Separation of Amino Acids by Capillary Electrophoresis with Light-emitting Diode-induced Fluorescence in the Presence of Electroosmotic Flow -- Quantification of Arginine and Dimethylated Arginines in Human Plasma by Field-amplified Sample Injection Capillary Electrophoresis UV Detection -- Capillary Electrophoresis-Mass Spectrometry for Peptide Analysis: Target-Based Approaches and Proteomics/Peptidomics Strategies -- High Resolution Proteome/peptidome Analysis of Body Fluids by Capillary Electrophoresis Coupled with MS -- Contribution of CE to the Analysis of Protein or Peptide Biomarkers -- Highly Charged Polyelectrolyte Coatings to Prevent Adsorption During Protein and Peptide Analysis in Capillary Electrophoresis -- Capillary Electrophoresis with Laser Induced Fluorescence Detection of Proteins from Two Types of Complex Sample Matrices: Food and Biological Fluids -- Automated Capillary Electrophoresis in the Screening for Haemoglobinopathies -- Protein Fingerprinting of Staphylococcus aureus by Capillary Electrophoresis with On-capillary Derivatization and Laser-induced Fluorescence Detection -- Capillary Electrophoresis of Seed Storage Proteins: The Separation and Identification of Microheterogeneous Rice Glutelin Subunits -- Evaluating Amyloid Beta (Ab) 1-40 Degradation by Capillary Electrophoresis -- Capillary Electrophoresis for Protein Profiling of the Dimorphic, Pathogenic Fungus, Penicillium marneffei -- Capillary Electrophoretic Enzyme Assays -- A Methodology for Detection and Quantification of Esterase Activity -- Enzyme Inhibitor Screening by CE with an on-Column Immobilized Enzyme Microreactor Created by an Ionic Binding Technique -- Fluorescent Lipids as Probes for Sphingosine Kinase Activity by Capillary Electrophoresis -- Assessment of DNA damage by Micellar Electrokinetic Chromathography.Approaches to Enhancing the Sensitivyty of Carbohydrate Separations in Capillary Electrophoresis -- Determination of Monosaccharides Derivatized with 2-Aminobenzoic Acid by Capillary Electrophoresis -- Determination of Mono- Di- and Oligosaccharides by Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection -- Separation of Chitooligosaccharides in Acidic Solution by Capillary Electrophoresis -- Capillary Electrophoresis for the Analysis of Glycosaminoglycan-derived Disaccharides -- High Throughput Capillary Electrophoresis Mass Spectrometry: From Analysis of Amino Acids to Analysis of Protein Complexes -- Separation of Amino Acids by Capillary Electrophoresis with Light-emitting Diode-induced Fluorescence in the Presence of Electroosmotic Flow -- Quantification of Arginine and Dimethylated Arginines in Human Plasma by Field-amplified Sample Injection Capillary Electrophoresis UV Detection -- Capillary Electrophoresis-Mass Spectrometry for Peptide Analysis: Target-Based Approaches and Proteomics/Peptidomics Strategies -- High Resolution Proteome/peptidome Analysis of Body Fluids by Capillary Electrophoresis Coupled with MS -- Contribution of CE to the Analysis of Protein or Peptide Biomarkers -- Highly Charged Polyelectrolyte Coatings to Prevent Adsorption During Protein and Peptide Analysis in Capillary Electrophoresis -- Capillary Electrophoresis with Laser Induced Fluorescence Detection of Proteins from Two Types of Complex Sample Matrices: Food and Biological Fluids -- Automated Capillary Electrophoresis in the Screening for Haemoglobinopathies -- Protein Fingerprinting of Staphylococcus aureus by Capillary Electrophoresis with On-capillary Derivatization and Laser-induced Fluorescence Detection -- Capillary Electrophoresis of Seed Storage Proteins: The Separation and Identification of Microheterogeneous Rice Glutelin Subunits -- Evaluating Amyloid Beta (Ab) 1-40 Degradation by Capillary Electrophoresis -- Capillary Electrophoresis for Protein Profiling of the Dimorphic, Pathogenic Fungus, Penicillium marneffei -- Capillary Electrophoretic Enzyme Assays -- A Methodology for Detection and Quantification of Esterase Activity -- Enzyme Inhibitor Screening by CE with an on-Column Immobilized Enzyme Microreactor Created by an Ionic Binding Technique -- Fluorescent Lipids as Probes for Sphingosine Kinase Activity by Capillary Electrophoresis -- Assessment of DNA damage by Micellar Electrokinetic Chromathography.
520 _aCapillary electrophoresis (CE) is a relatively new separation technique suitable for handling small amounts of sample very important in bioanalytical research and in various clinical, diagnostic, genetic, and forensic applications. In Capillary Electrophoresis of Biomolecules: Methods and Protocols, expert researchers in the field provide key techniques to investigate CE focusing on simple and complex carbohydrates (polysaccharides), aminoacids, peptides and proteins, enzymes, and nucleic acids. Along with practical procedures, reviews discussing CE applications related to bio(macro)molecules are also included. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Capillary Electrophoresis of Biomolecules: Methods and Protocols provides the reader with the latest break throughs and improvements in CE and CE techniques applied to several classes of bio(macro)molecules.
988 _aSpringer_Protocols_2013
650 7 _2embne
_9688491
_aElectroforesis capilar
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781627032971
776 0 8 _iPrinted edition:
_z9781627032957
776 0 8 _iPrinted edition:
_z9781493962488
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-296-4
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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_cLE
998 _b12/2023
_dz
_eb
_zSI