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008			100301s2006 xxua o |||| 0|eng d
020			_a9781592599936
024	7		_a10.1007/978-1-59259-993-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH585 _b2006 EB
245	0	0	_aCell Imaging Techniques _cedited by Douglas J. Taatjes, Brooke T. Mossman
250			_a1st edition 2006
264		1	_aTotowa, NJ _bHumana Press _c2006
300			_a1 recurso en línea (XIV, 490 páginas) _b212 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v319
505	0		_aMolecular Beacons -- Second-Harmonic Imaging of Collagen -- Visualizing Calcium Signaling in Cells by Digitized Wide-Field and Confocal Fluorescent Microscopy -- Multifluorescence Labeling Techniques and Confocal Laser Scanning Microscopy on Lung Tissue -- Evaluation of Confocal Microscopy System Performance -- Quantitative Analysis of Atherosclerotic Lesion Composition in Mice -- Applications of Microscopy to Genetic Therapy of Cystic Fibrosis and Other Human Diseases -- Laser Scanning Cytometry -- Near-Clinical Applications of Laser Scanning Cytometry -- Laser Capture Microdissection -- Analysis of Asbestos-Induced Gene Expression Changes in Bronchiolar Epithelial Cells Using Laser Capture Microdissection and Quantitative Reverse Transcriptase-Polymerase Chain Reaction -- New Approaches to Fluorescence In Situ Hybridization -- Microarray Image Scanning -- Near-Field Scanning Optical Microscopy in Cell Biology and Cytogenetics -- Porosome -- Secretory Vesicle Swelling by Atomic Force Microscopy -- Imaging and Probing Cell Mechanical Properties With the Atomic Force Microscope -- Reflection Contrast Microscopy -- Three-Dimensional Analysis of Single Particles by Electron Microscopy -- Three-Dimensional Reconstruction of Single Particles in Electron Microscopy -- A New Microbiopsy System Enables Rapid Preparation of Tissue for High-Pressure Freezing.
520			_aCell imaging methodologies have now become essential research tools for a variety of disciplines that traditionally had not relied on them. In Cell Imaging Techniques: Methods and Protocols, distinguished international researchers describe in detail their state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complementary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Timely and highly practical, Cell Imaging Techniques: Methods and Protocols provides researchers and clinicians with a richly useful guide to selecting and performing the best imaging method from a bewildering variety of microscopy-based techniques.
988			_aSpringer_Protocols_2006
650		7	_2embne _9301792 _aCitología _xTécnica
776	0	8	_iPrinted edition: _z9781617373947
776	0	8	_iPrinted edition: _z9781588291578
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59259-993-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI