000			04231nam a2200385 i 4500
999			_c393684 _d393684
001			393684
003			ES-MaUEC
005			20231227210342.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			100301s2006 xxu| o |||| 0|eng d
020			_a9781597450768
024	7		_a10.1385/1597450766 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP519.9 .C36 _b2006 EB
245	0	0	_aMicrochip Capillary Electrophoresis : _bMethods and Protocols _cedited by Charles Henry
250			_a1st edition 2006
264		1	_aTotowa, NJ _bHumana Press _c2006
300			_a1 recurso en línea (X, 238 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v339
505	0		_aMicrochip Capillary Electrophoresis -- Microchip Capillary Electrophoresis -- Microchip Fabrication Methods -- Fabrication of a Glass Capillary Electrophoresis Microchip With Integrated Electrodes -- Micro-Molding for Poly(dimethylsiloxane) Microchips -- Fabrication of Polymer Microfluidic Systems by Hot Embossing and Laser Ablation -- Surface Modification Methods -- Surface Modification Methods for Enhanced Device Efficacy and Function -- Polyelectrolyte Coatings for Microchip Capillary Electrophoresis -- Detection Methods for Microchip Capillary Electrophoresis -- Interfacing Microchip Capillary Electrophoresis With Electrospray Ionization Mass Spectrometry -- Interfacing Amperometric Detection With Microchip Capillary Electrophoresis -- Conductivity Detection on Microchips -- Applications of Microchip Capillary Electrophoresis -- DNA Separations -- Protein Separations -- Microchip Capillary Electrophoresis -- Measuring Electroosmotic Flow in Microchips and Capillaries -- Single Cell Analysis on Microfluidic Devices -- Rapid DNA Amplification in Glass Microdevices.
520			_aMicrochip capillary electrophoresis, a new and emerging technology that can rapidly analyze biological compounds as small as or smaller than a single cell, will have a major impact on biological sciences in the next 10 to 20 years. In Microchip Capillary Electrophoresis: Methods and Protocols, leading chemists and engineers concisely explain the principles behind this revolutionary technique and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. The authors describe in detail the detection methods used, including mass spectrometry, electrochemistry, and conductivity. Since surface chemistry must be carefully considered for optimal operation at this scale, they also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Instructive and well-detailed, Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for both initiating research in the field of microchip capillary electrophoresis and understanding the full range of how these new systems can be used successfully in biological research.
988			_aSpringer_Protocols_2006
650		7	_2embne _9688491 _aElectroforesis capilar _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617374760
776	0	8	_iPrinted edition: _z9781588292933
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1597450766 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI