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008			120507s2012 xxua o |||| 0|eng d
020			_a9781617798603
024	7		_a10.1007/978-1-61779-860-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQM311 _b2012 EB
245	0	0	_aOdontogenesis : _bMethods and Protocols _cedited by Chrissa Kioussi
250			_a1st edition 2012
264		1	_aTotowa, NJ _bHumana Press _c2012
300			_a1 recurso en línea (X, 194 páginas) _b40 ilustraciones, 24 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v887
505	0		_aHistological Analysis of the Embryonic and Adult Tooth -- Determination of Gene Expression Patterns by Whole-Mount In Situ Hybridization -- Determination of Gene Expression Patterns by In Situ Hybridization in Sections -- Immunohistochemistry and Detection of Proliferating Cells by BrdU -- Detection of Apoptosis by TUNEL Assay -- Use of siRNA in Dental Tissue-Derived Cell Cultures: Integrin Knockdown in Fibroblasts -- Organ Cultures and Kidney-Capsule Grafting of Tooth Germs -- Evaluation of Skull and Tooth Morphology and Mineralization Using High-Resolution X-Ray Tomography -- Electron Microscopy -- Deoxyoligonucleotide Microarrays for Gene Expression Profiling in Murine Tooth Germs -- Lineage Differentiation of Mesenchymal Stem Cells from Dental Pulp, Apical Papilla, and Periodontal Ligament -- In Vivo Transplantation and Tooth Repair -- Methods to Validate Tooth-Supporting Regenerative Therapies -- Generation of a Bioengineered Tooth by Using a Three-Dimensional Cell Manipulation Method (Organ Germ Method) -- In Vitro Studies on Odontogenic Tumors -- Whole Mount Immunohistochemistry and In Situ Hybridization of Larval and Adult Zebrafish Dental Tissues.
520			_aOnly in recent times has the possibility of growing and implanting replacement teeth, made from one's own cells, moved into the realm of realistic possibilities; however, the molecular and cellular mechanisms of tooth development must be studied in a range of vertebrates, from zebrafish to mice, so that evolutionarily conserved network kernels, which will define the cellular states of generic vertebrate tooth development, can be recognized.  In Odontogenesis: Methods and Protocols, experts in the field examine techniques to approach this burgeoning field.  This detailed volume includes chapters on the detection of "tooth development" gene expression, both at the RNA and protein level, current approaches to the manipulation of gene expression levels and subsequent analysis of tooth phenotypes, as well as chapters concerning current efforts to get living tooth implants working without waiting for a full understanding of the developmental pathways at the molecular level.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for trouble-shooting and avoiding known pitfalls.   Practical and easy to use, Odontogenesis: Methods and Protocols aims to help researchers move forward toward the ultimate goal of getting a "bioengineered tooth" into the patient's mouth.
988			_aSpringer_Protocols_2012
650		7	_2embne _9179726 _aDientes _xAnatomía _vManuales de laboratorio
650		7	_2embne _9165020 _aDentición _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617798597
776	0	8	_iPrinted edition: _z9781617798610
776	0	8	_iPrinted edition: _z9781493959471
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-860-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI