000			03918nam a2200409 i 4500
999			_c393682 _d393682
001			393682
003			ES-MaUEC
005			20231227204838.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			141118s2015 xxu| o |||| 0|eng d
020			_a9781493918621
024	7		_a10.1007/978-1-4939-1862-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH465 .A1 _b2015 EB
245	0	0	_aChromosomal Mutagenesis _cedited by Shondra M. Pruett-Miller
250			_a2nd edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XIV, 333 páginas) _b22 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1239
505	0		_aGenome Editing by Targeted Chromosomal Mutagenesis -- ­piggyBac Transposon-Based Insertional Mutagenesis in Mouse Haploid Embryonic Stem Cells -- Using Phage Integrases in a Site-Specific Dual Integrase Cassette Exchange Strategy -- Genome Mutagenesis Using Synthetic Triplex-Forming Molecules and Recombinagenic DNA -- Genome Engineering Using Adeno-Associated Virus (AAV) -- Engineering of Customized Meganucleases Via In Vitro Compartmentalization and In Cellulo Optimization -- Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform -- Ligation-Independent Cloning (LIC) Assembly of TALENs -- Assembly and Characterization of megaTALs for Hyperspecific Genome Engineering Applications -- Genome Engineering Using CRISPR-Cas9 System -- Donor Plasmid Design for Codon and Single Base Genome Editing Using Zinc Finger Nucleases -- Endogenous Gene Tagging with Fluorescent Proteins -- Silencing Long Non-Coding RNAs with Genome-Editing Tools -- Gene Editing Using ssODNs with Engineered Endonucleases -- Genome Editing in Human Pluripotent Stem Cells Using Site-Specific Nucleases -- Strategies to Increase Genome Editing Frequencies and to Facilitate the Identification of Edited Cells -- Using Engineered Endonucleases to Create Knockout and Knockin Zebrafish Models -- Creating Knockout and Knockin Rodents Using Engineered Endonucleases Via Direct Embryo Injection -- Simple Sperm Preservation by Freeze-Drying for Conserving Animal Strains.
520			_aThis new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols. Since the first edition, major advances and discoveries have made chromosomal mutagenesis a widely used technique and one that is available to any molecular biology laboratory, and this collection provides detailed protocols, case-studies, and reviews from thought-leaders in the field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, Chromosomal Mutagenesis, Second Edition aims to help speed scientific discovery and aid in the next advances in the field.
988			_aSpringer_Protocols_2015
650		7	_2embne _9156720 _aMutagénesis
650		7	_2embne _9144875 _aMutación (Biología)
776	0	8	_iPrinted edition: _z9781493918638
776	0	8	_iPrinted edition: _z9781493918614
776	0	8	_iPrinted edition: _z9781493955411
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-1862-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI