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020 _a9781627034111
024 7 _a10.1007/978-1-62703-411-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aQP625 .N89
_b2013 EB
245 0 0 _aTrinucleotide Repeat Protocols
_cedited by Yoshinori Kohwi, Cynthia T. McMurray
250 _a2nd edition 2013
264 1 _aTotowa, NJ
_bHumana Press
_c2013
300 _a1 recurso en línea (XIII, 296 páginas)
_b64 ilustraciones, 24 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1010
505 0 _aA Brief History of Triplet Repeat Disease -- Huntington's Disease and Cell Therapies: Past, Present and Future -- Transcranial Two-Photon Imaging of Synaptic Structures in the Cortex of Awake Head-Restrained Mice -- In vivo Imaging of Synapse Plasticity in the Mouse Motor Cortex -- Pluripotent Hybrid Stem Cells from Transgenic Huntington's Disease Monkey -- Mesenchymal Stem Cells for Trinucleotide Repeat Disorders -- Lentiviral-Mediated Gene Transfer of siRNAs for the Treatment of Huntington's Disease -- Purification of dFMR1-Containing Complexes Using Tandem Affinity Purification -- Combined FISH and Immunofluorescent Staining Methods to Co-localize Proteins and mRNA in Neurons and Brain Tissue -- Epigenetic Modifications of the FMR1 Gene -- The CGG Repeat and the FMR1 Gene -- Study of Oxidative Damage and Antioxidant Systems in Two Huntington´s Disease Rodent Models -- Regulation of Ataxin-1 Phosphorylation and Its Impact on Biology -- Towards Understanding Region-Specificity of Triplet Repeat Diseases: Coupled Immunohistology and Mass Spectrometry Imaging -- Antibodies and Intrabodies Against Huntingtin: Production and Screening of Monoclonals and Single-Chain Recombinant Forms -- Cell Recovery from DM1 Transgenic Mouse Tissue to Study (CTG)n Instability and DM1 Pathogenesis -- Markerless Modification of Trinucleotide Repeat Loci in BACs -- DRPLA: Recent Advances in Research Using Transgenic Mouse Models.
520 _aTwo decades have passed since trinucleotide repeat expansion was first discovered in genes responsible for certain neurological diseases. Since then, new technologies have developed and innovative concepts have emerged, which may prove useful in devising therapeutic approaches to neurological diseases. Divided into six convenient sections, Trinucleotide Repeat Protocols, Second Edition covers a wide range of topics such as an overview of trinucleotide repeat diseases, synaptic plasticity, embryonic stem (ES) cell-related protocols with a focus on HD, RNA-related protocols, and analysis of epigenetic modification in fragile X syndrome.  This edition focuses not only on direct analysis of trinucleotide repeat diseases but also on alternative approaches for the analysis of trinucleotide repeat diseases, with the hope that this will result in a better understanding of the mechanisms and future therapeutic prospects for treatment of these diseases. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Trinucleotide Repeat Protocols, Second Edition seeks to serve researchers with its thorough methodologies on this expanding field.
988 _aSpringer_Protocols_2013
650 7 _2embne
_9144345
_aNucleótidos
776 0 8 _iPrinted edition:
_z9781627034128
776 0 8 _iPrinted edition:
_z9781627034104
776 0 8 _iPrinted edition:
_z9781493959716
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-411-1
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b01/2024
_dz
_eb
_zSI