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008			100301s2008 xxua o |||| 0|eng d
020			_a9781597455794
024	7		_a10.1007/978-1-59745-579-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2008 EB
245	0	0	_aAvidin-Biotin Interactions : _bMethods and Applications _cedited by Robert J. McMahon
250			_a1st edition 2008
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XIV, 232 páginas) _b38 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v418
505	0		_aPreparation of Avidin Conjugates -- Coupling of Antibodies With Biotin -- Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abundance Using Avidin and Streptavidin -- Use of the Avidin (Imino)Biotin System as a General Approach to Affinity Precipitation -- Capturing Biotinylated Proteins and Peptides by Avidin Functional Affinity Electrophoresis -- Functionality Screen of Streptavidin Mutants by Non-Denaturing SDS-PAGE Using Biotin-4-Fluorescein -- Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and Biotin or Biotin Derivatives -- Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resins -- High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed E. coli -- Endogenous Biotin in Rat Brain -- Pseudo-Immunolabelling With the Avidin-Biotin-Peroxidase Complex due to the Presence of Endogenous Biotin in the Retina -- Use of Synthetic Peptides for Identifying Biotinylation Sites in Human Histones -- Detection and Quantitation of the Activity of DNA Methyltransferases Using a Biotin/Avidin Microplate Assay -- Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin -- Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedure -- A Multiplex Approach to Isotyping Antigen-Specific Antibodies Using Biotinylated Antigen/Streptavidin-Phycoerythrin -- Biotin-Protein Bond: Instability and Structural Modification to Provide Stability for In Vivo Applications.
520			_aFor researchers today, the avidin-biotin interaction is among the most widely exploited among biomedical scientists. Despite its popularity, avidin-biotin methodology suffers from a lack of clarity associated with popular, low cost, prefabricated kits used by many in the field. In Avidin-Biotin Interactions: Methods and Applications, leaders in avidin-biotin methodology share their knowledge relating to the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. Although avidin-biotin based kits based have been produced for quite some time and remain a widely used methodology, many of the chapters in this text describe situations in which the previous methodology failed due to some factor, and the authors developed new and better ways for the avidin-biotin system to be utilized. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist. Each chapter in Avidin-Biotin Interactions: Methods and Applications contains not only excellent descriptions of protocols for the lab but also the experience of researchers who used specific methodology, found a problem with that methodology in a new setting, and ultimately devised ways to minimize or obviate the limitations of the technology.
988			_aSpringer_Protocols_2008
650		7	_2embne _9139861 _aProteínas _vManuales de laboratorio
700	1		_aMcMahon, Robert J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617376573
776	0	8	_iPrinted edition: _z9781588295835
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-579-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI