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008			100301s2007 xxua o |||| 0|eng d
020			_a9781597455282
024	7		_a10.1007/978-1-59745-528-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP601 _b2007 EB
245	0	0	_aPCR Primer Design _cedited by Anton Yuryev
250			_a1st edition 2007
264		1	_aTotowa, NJ _bHumana Press _c2007
300			_a1 recurso en línea (XIV, 432 páginas) _b128 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v402
505	0		_aBasic Principles and Software for PCR Primer Design -- Physical Principles and Visual-OMP Software for Optimal PCR Design -- OLIGO 7 Primer Analysis Software -- Selection for 3?-End Triplets for Polymerase Chain Reaction Primers -- The Reference Point Method in Primer Design -- PCR Primer Design Using Statistical Modeling -- Developing a Statistical Model for Primer Design -- Genome-Scale PCR Primer Design -- GST-PRIME -- Genome-Scale Probe and Primer Design with PRIMEGENS -- Repeat Masking for PCR Primer Design -- SNPbox -- Fast Masking of Repeated Primer Binding Sites in Eukaryotic Genomes -- Multiplex PCR Primer Design -- Degenerate Primer Design -- An Iterative Method for Selecting Degenerate Multiplex PCR Primers -- Primer Design for Multiplexed Genotyping -- MultiPLX -- MultiPrimer -- Allele-specific PCR -- Modified Oligonucleotides as Tools for Allele-Specific Amplification -- AlleleID -- Long PCR Primer Design -- Designing Primers for Whole Genome PCR Scanning Using the Software Package GenoFrag -- DNA Methylation Mapping -- Designing PCR Primer for DNA Methylation Mapping -- BiSearch -- Graphical Design of Primers with PerlPrimer.
520			_aIn the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as transcriptome, proteome, metabolome and other -omes. At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). PCR Primer Design focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, this volume is an exceptional reference for all those involved in studying the genome. In PCR Primer Design, authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome-scale experiments and for small-scale individual PCR amplifications. This volume will be useful for organizations performing whole genome studies, companies designing instruments that utilize PCR, and individual scientists - geneticists, molecular biologists, molecular geneticists, and more - who routinely use PCR in their research.
988			_aSpringer_Protocols_2007
650		7	_2embne _9138640 _aEnzimas
776	0	8	_iPrinted edition: _z9781617377433
776	0	8	_iPrinted edition: _z9781588297259
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-528-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI