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008			131203s2014 xxua o |||| 0|eng d
020			_a9781627037334
024	7		_a10.1007/978-1-62703-733-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTP248.2 _b2014 EB
245	0	0	_aAnimal Cell Biotechnology : _bMethods and Protocols _cedited by Ralf Pörtner
250			_a3rd edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (XIV, 498 páginas) _b83 ilustraciones, 28 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1104
505	0		_aHigh-Throughput Synchronization of Mammalian Cell Cultures by Spiral Microfluidic Sorter -- Insect Cell Line Development Using Flp-Mediated Cassette Exchange Technology -- Scalable Transient Gene Expression in Adherent Mammalian Cells Using Polyethylenimine -- Scalable Transient Protein Expression -- DNA Profiling and Characterisation of Animal Cell Lines -- Tools for High-Throughput Process and Medium Optimization -- Designing Media for Animal Cell Culture - CHO Cells, The Industrial Standard -- Feed Optimization in Fed-Batch Culture -- Screening and Optimization of Chemically-Defined Media and Feeds with Integrated and Statistical Approaches -- Evaluation of Solid and Porous Microcarriers for Cell Growth and Production of Recombinant Proteins -- Microbioreactors and Scale-Down Models: Growth of CHO Cells Using the Pall Micro24 MicroReactor System -- Monitoring Cell Growth, Viability and Apoptosis -- Metabolic Flux Estimation in Mammalian Cell Cultures -- Quenching Methods for the Analysis of Intracellular Metabolites -- NMR Methods for Metabolomics of Mammalian Cell Culture Bioreactors -- 1H-NMR Protocol for Exometabolome Analysis of Cultured Mammalian Cells -- Microfluidic Perfusion Culture -- Disposable Bioreactors for Inoculum Production and Protein Expression -- Configuration of Bioreactors -- Process Analytical Technology (PAT) in Insect and Mammalian Cell Culture Processes - Dielectric Spectroscopy and Focused Beam Reflectance Measurement (FBRM) -- Cell Assessment by Atline Microscopy -- Seed Train Optimization for Cell Culture -- Vaccine Production - Upstream Processing with Adherent or Suspension Cell Lines -- Efficient Processes for Protein Expression Using Recombinant Baculovirus Particles -- Chromatographic Techniques in the Downstream Processing of Proteins in Biotechnology -- Virus Separation Using Membranes.
520			_aAnimal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
988			_aSpringer_Protocols_2014
650		7	_2embne _9140931 _aBiotecnología _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781627037327
776	0	8	_iPrinted edition: _z9781627037341
776	0	8	_iPrinted edition: _z9781493963188
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-733-4 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI