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008			160219s2016 xxua o |||| 0|eng d
020			_a9781493933723
024	7		_a10.1007/978-1-4939-3372-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH440.5 _b2016 EB
245	0	0	_aTransposons and Retrotransposons : _bMethods and Protocols _cedited by Jose Luis Garcia Perez
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XII, 434 páginas) _b73 ilustraciones, 56 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1400
505	0		_aStudy of Transposable Elements and their Genomic Impact -- Bacterial Group II Introns: Identification and Mobility Assay -- In silico Methods to Identify Exapted Transposable Element Families -- Retrotransposon Capture Sequencing (RC-seq): A Targeted, High-Throughput Approach to Resolve Somatic L1 Retrotransposition in Humans -- Long Interspersed Element Sequencing (L1-Seq): A Method to Identify Somatic LINE-1 Insertions in the Human Genome -- Combining Amplification Typing of L1 Active Subfamilies (ATLAS) with High Throughput Sequencing -- RNA-seq Analysis to Measure the Expression of SINE Retroelements -- Qualitative and Quantitative Assays of Transposition and Homologous Recombination of the Retrotransposon Tf1 in Schizosaccharomyces pombe -- LINE Retrotransposition Assays in Saccharomyces cerevisiae -- LINE-1 Cultured Cell Retrotransposition Assay -- L1 Retrotransposition in Neural Progenitor Cells -- Characterization of Engineered L1 Retrotransposition Events: The Recovery Method -- SINE Retrotransposition: Evaluation of Alu Activity and Recovery of de novo Inserts -- The Engineered SVA Trans-Mobilization Assay -- Detection of LINE-1 RNAs by Northern Blot -- Monitoring Long Interspersed Nuclear Element 1 Expression during Mouse Embryonic Stem Cells Differentiation -- Immuno-Detection of Human LINE-1 Expression in Cultured Cells and Human Tissues -- Cellular Localization of Engineered Human LINE-1 RNA and Proteins -- Purification of L1-ribonucleoprotein Particles (L1-RNPs) from Cultured Human Cells -- Characterization of L1-RiboNucleoprotein Particles -- LEAP: L1 Element Amplification Protocol -- Biochemical Approaches to Study LINE-1 Reverse Transcriptase Activity In Vitro -- Methylated DNA Immunoprecipitation Analysis of Mammalian Endogenous Retroviruses -- Profiling DNA Methylation and Hydroxymethylation at Retrotransposable Elements -- A Large-Scale Functional Screen to Identify Epigenetic Repressors of Retrotransposon Expression -- Reprogramming of Human Fibroblasts to Induced Pluripotent Stem Cells with Sleeping Beauty Transposon-based Stable Gene Delivery. .
520			_aThis volume covers the latest protocols designed to identify and characterize TEs in genomes, ancient or recently inserted. Additionally, this book includes a series of protocols designed to understand how host genomes act to regulate the activity of TEs, from elegant genetic mobilization assays to key biochemical methods. Finally, this book also includes chapters that describe how TEs can be used for biotechnological applications. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Transposons and Retrotransposons: Methods and Protocols aims to ensure successful results in the further study of this vital field.
988			_aSpringer_Protocols_2016
650		7	_2embne _9138044 _aGenética _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493933709
776	0	8	_iPrinted edition: _z9781493933716
776	0	8	_iPrinted edition: _z9781493980338
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3372-3 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI