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008			140106s2014 xxua o |||| 0|eng d
020			_a9781627037648
024	7		_a10.1007/978-1-62703-764-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH442.2 _b2014 EB
245	0	0	_aDNA Cloning and Assembly Methods _cedited by Svein Valla, Rahmi Lale
250			_a1st edition 2014
264		1	_aTotowa, NJ _bHumana Press _c2014
300			_a1 recurso en línea (XI, 303 páginas) _b74 ilustraciones, 50 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1116
505	0		_aBioBrick Assembly Standards and Techniques and Associated Software Tools -- Plasmid Construction by SLIC or Sequence and Ligation Independent Cloning -- Quick and Clean Cloning -- Hierarchical Ligation-Independent Assembly of PCR Fragments -- USER-Derived Cloning Methods and Their Primer Design -- Application of the Restriction-Free (RF) Cloning for Multi-Components Assembly -- A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform -- Circular Polymerase Extension Cloning -- Golden Gate Cloning -- Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy -- FX cloning: A Simple and Robust High-Throughput Cloning Method for Protein Expression -- Minimum GC-rich Sequences for Overlap Extension PCR and Primer Annealing -- Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR -- Combinatorial Assembly of Clone Libraries Using Site-Specific Recombination -- Application of In-Fusion™ Cloning for the Parallel Construction of Escherichia coli Expression Vectors -- SeamLess Ligation Cloning Extract（SLiCE）Cloning Method -- j5 DNA Assembly Design Automation -- FASTPCR Software for PCR, in silico PCR, and Oligonucleotide Assembly and Analysis.
520			_aIn DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination. With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide  scientist with a valuable and useful resource for wet lab researchers within life sciences.
988			_aSpringer_Protocols_2014
650		7	_2embne _9159969 _aClonación molecular
776	0	8	_iPrinted edition: _z9781627037655
776	0	8	_iPrinted edition: _z9781627037631
776	0	8	_iPrinted edition: _z9781493960576
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-764-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI