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008			101013s2011 xxua o |||| 0|eng d
020			_a9781607616825
024	7		_a10.1007/978-1-60761-682-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK981 _b2011 EB
245	0	0	_aPlant Reverse Genetics : _bMethods and Protocols _cedited by Andy Pereira
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XI, 282 páginas) _b63 ilustraciones, 2 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v678
505	0		_aAnalysis of High-Throughput Sequencing Data -- Identification of Plant MicroRNAs Using Expressed Sequence Tag Analysis -- Microarray Data Analysis -- Setting Up Reverse Transcription Quantitative-PCR Experiments -- Virus-Induced Gene Silencing in Nicotiana benthamiana and Other Plant Species -- Agroinoculation and Agroinfiltration: Simple Tools for Complex Gene Function Analyses -- Full-Length cDNA Overexpressor Gene Hunting System (FOX Hunting System) -- Activation Tagging with En/Spm-I/dSpm Transposons in Arabidopsis -- Activation Tagging and Insertional Mutagenesis in Barley -- Methods for Rice Phenomics Studies -- Development of an Efficient Inverse PCR Method for Isolating Gene Tags from T-DNA Insertional Mutants in Rice -- Transposon Insertional Mutagenesis in Rice -- Reverse Genetics in Medicago truncatula Using Tnt1 Insertion Mutants -- Screening Arabidopsis Genotypes for Drought Stress Resistance -- Protein Tagging for Chromatin Immunoprecipitation (ChIP) from Arabidopsis -- Yeast One-Hybrid Screens for Detection of Transcription Factor DNA Interactions -- Plant Metabolomics by GC-MS and Differential Analysis -- Gramene Database: A Hub for Comparative Plant Genomics.
520			_aAfter the generation of genome sequence data from a wide variety of plants, databases are filled with sequence information of genes with no known biological function, and while bioinformatics tools can help analyze genome sequences and predict gene structures, experimental approaches to discover gene functions need to be widely implemented. In Plant Reverse Genetics: Methods and Protocols, leading researchers in the field describe cutting-edge methods, both high-throughput and genome-wide, involving the models Arabidopsis and rice as well as several other plants to provide comparative functional genomics information. With chapters on the analysis of high-throughput genome sequence data, the identification of non-coding RNA from sequence information, the comprehensive analysis of gene expression by microarrays, and metabolomic analysis, the thorough methods of the book are fully supported by scripts to aid their computational use. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Plant Reverse Genetics: Methods and Protocols is an ideal guide for researchers seeking an understanding of how the complex web of plant genes work together in a systems biology view.
988			_aSpringer_Protocols_2011
650		7	_2embne _9139640 _aGenética vegetal
776	0	8	_iPrinted edition: _z9781607616832
776	0	8	_iPrinted edition: _z9781607616818
776	0	8	_iPrinted edition: _z9781493961276
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-682-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI