000			05129nam a2200409 i 4500
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003			ES-MaUEC
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007			cr nn 008mamaa
008			100301s2009 xxua o |||| 0|eng d
020			_a9781597454506
024	7		_a10.1007/978-1-59745-450-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR186.5 _b2009 EB
245	0	0	_aEpitope Mapping Protocols _cedited by Ulrich Reineke, Mike Schutkowski
250			_a2nd edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XIV, 450 páginas) _b85 ilustraciones, 19 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v524
505	0		_aB-cell Epitope Mapping -- What Is a B-Cell Epitope? -- Whole Antigen Methods -- Structural Basis of Antibody-Antigen Interactions -- Epitope Mapping of Antibody-Antigen Complexes by Nuclear Magnetic Resonance Spectroscopy -- A Solid-Phase Mutual Inhibition Assay with Labeled Antigen -- Epitope Mapping by Surface Plasmon Resonance -- Proteolytic Fragmentation for Epitope Mapping -- Epitope Mapping by Proteolysis of Antigen-Antibody Complexes -- Identifying Residues in Antigenic Determinants by Chemical Modification -- Epitope Mapping by Differential Chemical Modification of Antigens -- Peptide Library Approaches -- Linear B-Cell Epitope Mapping Using Enzyme-Linked Immunosorbent Assay for Libraries of Overlapping Synthetic Peptides -- Antibody Epitope Mapping Using SPOT™ Peptide Arrays -- Peptide Microarrays for Profiling of Modification State-Specific Antibodies -- Epitope Mapping Using Phage Display Peptide Libraries -- Antibody Epitope Mapping Using De Novo Generated Synthetic Peptide Libraries -- Antibody Specificity Profiling on Functional Protein Microarrays -- Peptide Microarrays for Determination of Cross-Reactivity -- Profiling of Antibody Signatures in Biological Fluids. -- Epitope Mapping Using Randomly Generated Peptide Libraries -- Probing the Epitope Signatures of IgG Antibodies in Human Serum from Patients with Autoimmune Disease -- Microarrayed Allergen Molecules for Diagnostics of Allergy -- Monitoring B Cell Response to Immunoselected Phage-Displayed Peptides by Microarrays -- Antigen Expressed from Recombinant DNA -- Epitope Mapping Using Homolog-Scanning Mutagenesis -- Epitope Mapping by Region-Specified PCR-Mutagenesis -- Epitope Mapping Using Phage-Display Random Fragment Libraries -- B-Cell Epitope Prediction -- Prediction of Linear B-cell Epitopes -- T-Cell Epitope Mapping -- Molecular Recognition of Diverse Ligands by T-Cell Receptors -- Identification of Human MHC Class I Binding Peptides using the iTOPIA™? Epitope Discovery System -- T-Cell Epitope Mapping in Mycobacterium tuberculosis Using PepMixes Created by Micro-Scale SPOT™? Synthesis -- High-Throughput T-Cell Epitope Discovery Through MHC Peptide Exchange -- T-Cell Epitope Processing (The Epitope Flanking Regions Matter) -- Identification of MHC Class II Binding Peptides: Microarray and Soluble MHC Class II Molecules -- T-Cell Epitope Mapping -- Identification and Validation of T-Cell Epitopes Using the IFN-? EliSpot Assay.
520			_aGiven the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping.
988			_aSpringer_Protocols_2009
650		7	_2embne _9138531 _aAntígenos
650		7	_2embne _9138330 _aInmunología
776	0	8	_iPrinted edition: _z9781607613534
776	0	8	_iPrinted edition: _z9781617379246
776	0	8	_iPrinted edition: _z9781934115176
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-450-6 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI