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008			100301s2000 xxu| o |||| 0|eng d
020			_a9781592590278
024	7		_a10.1007/978-1-59259-027-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aTP248.24 _b2000 EB
245	0	0	_aDownstream Processing of Proteins : _bMethods and Protocols _cedited by Mohamed A. Desai
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (IX, 229 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Biotechnology _x1940-607X _v9
505	0		_aDownstream Processing in the Biotechnology Industry -- 2 Microbial Cell Disruption by High-Pressure Homogenization -- 3 Protein Concentration and Buffer Exchange Using Ultrafiltration -- 4 High-Performance Tangential Flow Filtration for Protein Separations -- Large-Scale Recovery of Protein Inclusion Bodies by Continuous Centrifugation -- Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules -- Application of Chromatography in the Downstream Processing of Biomolecules -- Affinity Ligand Technology -- Immunoaffinity Adsorption in the Large-Scale Isolation of Biomolecules -- Expanded Bed Adsorption in the Purification of Biomolecules -- Application of HPLC in the Purification of Biomolecules -- Countercurrent Chromatography of Proteins with Polymer Phase Systems -- Practical Aspects and Applications of Radial Flow Chromatography -- Sterile Filtration and Virus Filtration -- Design and Interpretation of Viral Clearance Studies for Biopharmaceutical Products -- Validation of Sterilizing Filters in the Biotechnology Industry.
520			_aIn Downstream Processing of Proteins: Methods and Protocols, Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods with a potential for scale-up. Also treated are the various strands of the downstream biological process essential for a successful product license application, including both the validation of DSP stages, and the design and validation of viral clearance stages during the purification process. Downstream Processing of Proteins: Methods and Protocols provides scientists everywhere, but particularly in the biopharmaceutical and biotechnology industry, with a much-needed introduction to this critical technology. Every bioprocess scientist and engineer working to design and validate biological processes for novel proteins-and successfully apply for their new product licenses-will find this important book an eminently practical resource.
988			_aSpringer_Protocols_2000
650		7	_2embne _9140931 _aBiotecnología _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617370755
776	0	8	_iPrinted edition: _z9780896035645
776	0	8	_iPrinted edition: _z9781489941794
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59259-027-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI