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008			170317s2017 xxua o |||| 0|eng d
020			_a9781493969258
024	7		_a10.1007/978-1-4939-6925-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC596 _b2017 EB
245	0	0	_aFood Allergens : _bMethods and Protocols _cedited by Jing Lin, Marcos Alcocer
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (X, 299 páginas) _b44 ilustraciones, 20 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1592
505	0		_aOverview of the Commonly used Methods for Food Allergens -- Allergen Extraction and Purification from Natural Products: Main Chromatographic Techniques -- Recombinant Allergen Production in E. Coli -- Recombinant Allergens Production in Yeast -- 2D-Electrophoresis and Immunoblotting in Food Allergy -- Two Dimensional Electrophoresis and Identification by Mass Spectrometry (MS) -- Enzyme-Linked Immunosorbent Assay (ELISA) -- Detection of Food Allergens by Taqman Real-Time PCR Methodology -- Detection of Food Allergens by Phage Displayed Produced Antibodies -- Protein Microarray-Based IgE-Immunoassay for Allergy Diagnosis -- Basophil Degranulation Assay -- Use of Humanized RS-ATL8 Reporter System for Detection of Allergen-Specific IgE Sensitization in Human Food Allergy -- Assessment of IgE Reactivity of β-Casein by Western Blotting after Digestion with Simulated Gastric Fluid -- IgE Epitope Mapping using Peptide Microarray Immunoassay -- T Cell Proliferation Assay: Determination of Immunodominant T Cell Epitopes of Food Allergens -- Tetramer Guided Epitope Mapping: A Rapid Approach to Identify HLA-Restricted T-Cell Epitopes from Composite Allergens -- T-Cell Epitope Prediction -- An Overview of Bioinformatics Tools and Resources in Allergy -- The Use of a Semi-Automated System to Measure Mouse Natural Killer T (NKT) Cell Activation by Lipid Loaded Dendritic Cells -- Recent Advances in the Detection of Allergens in Foods.
520			_aThis volume provides a collection of methodologies for basic research, clinical diagnosis, and treatment pertaining to food allergens, including food allergen production, purification, characterization, detection, quantification, and bioinformatics approaches to modern food allergen studies. The chapters in the book are divided into 4 parts: Part I discusses food allergen purification and production, and explores methods of producing recombinant food allergens in bacterial and yeast expression systems; Part II looks at allergen discovery, detection, and quantification covering 3 types of methods-DNA-, protein-, and cell-based methods; Part III focuses on allergenic epitope mapping; and Part IV talks about future developments concentrated around new concepts of allergenicity as an outcome of protein and food matrix interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Food Allergens: Methods and Protocols is a valuable resource for immunologists, biochemists, molecular biologists, and medical doctors and students working in the food allergy field. This book is also useful for people in the food industry, legislators, food standard agencies, allergologists, pediatricians, and clinicians in the allergic diseases and immunology fields. .
988			_aSpringer_Protocols_2017
650		7	_2embne _9141654 _aAlergia a los alimentos _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493969234
776	0	8	_iPrinted edition: _z9781493969241
776	0	8	_iPrinted edition: _z9781493983407
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6925-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI