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008			100316s2010 xxua o |||| 0|eng d
020			_a9781607616702
024	7		_a10.1007/978-1-60761-670-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQC176.8 .P55 _b2010 EB
245	0	0	_aSurface Plasmon Resonance : _bMethods and Protocols _cedited by Nico J. de Mol, Marcel J. E. Fischer
250			_a1st edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (X, 255 páginas) _b77 ilustraciones, 6 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v627
505	0		_aSurface Plasmon Resonance: A General Introduction -- The Role of Mass Transport Limitation and Surface Heterogeneity in the Biophysical Characterization of Macromolecular Binding Processes by SPR Biosensing -- Amine Coupling Through EDC/NHS: A Practical Approach -- High-Affinity Immobilization of Proteins Using Biotin- and GST-Based Coupling Strategies -- A Capture Coupling Method for the Covalent Immobilization of Hexahistidine Tagged Proteins for Surface Plasmon Resonance -- Affinity Constants for Small Molecules from SPR Competition Experiments -- Surface Plasmon Resonance Signal Enhancement for Immunoassay of Small Molecules -- High-Throughput Kinase Assay Based on Surface Plasmon Resonance -- SPR Biosensor as a Tool for Screening Prion Protein Binders as Potential Antiprion Leads -- Carbohydrate-Lectin Interactions Assayed by SPR -- DNA Sensors Based on Mixed Self-Assembled DNA/Alkanethiol Films -- Preparation of Lipid Membrane Surfaces for Molecular Interaction Studies by Surface Plasmon Resonance Biosensors -- Capture of Intact Liposomes on Biacore Sensor Chips for Protein-Membrane Interaction Studies -- Surface Plasmon Resonance Spectroscopy for Studying the Membrane Binding of Antimicrobial Peptides -- Surface Plasmon Resonance Spectroscopy in Determination of the Interactions Between Amyloid ? Proteins (A?) and Lipid Membranes -- Incorporation of a Transmembrane Protein into a Supported 3D-Matrix of Liposomes for SPR Studies -- Application of Surface Plasmon Resonance Spectroscopy to Study G-Protein Coupled Receptor Signalling -- Integration of SPR Biosensors with Mass Spectrometry (SPR-MS) -- SPR/MS: Recovery from Sensorchips for Protein Identification by MALDI-TOF Mass Spectrometry.
520			_aWhile commercial instruments have expanded the usage and the related literature has increased, the quality of surface plasmon resonance (SPR) research has been hindered by a lack of knowledge of the processes that influence the SPR signal. In Surface Plasmon Resonance: Methods and Protocols, experts in the field present a wide variety of applications involving commercially available SPR instruments. The heart of the SPR technique is to construct a dedicated surface for an assay. Protocols describe such surfaces for many needs, including the study of membrane bound proteins. Beyond the protocols-based chapters, the volume also highlights the backgrounds of vital issues in the use of SPR, including processes occurring within the hydrogel environment of sensors and on lipid membrane surfaces as well as the analysis of kinetic information. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Surface Plasmon Resonance: Methods and Protocols fills a need for well-described, hands-on SPR experimental protocols and promises to inspire the adaptation of these techniques to fit the needs of labs around the world.
988			_aSpringer_Protocols_2010
650		7	_2embne _9672140 _aPlasma (Gases ionizados) _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781607616719
776	0	8	_iPrinted edition: _z9781607616696
776	0	8	_iPrinted edition: _z9781493961252
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-670-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI