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008			170123s2017 xxua o |||| 0|eng d
020			_a9781493966257
024	7		_a10.1007/978-1-4939-6625-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aRC685 .H8 _b2017 EB
245	0	0	_aHypertension : _bMethods and Protocols _cedited by Rhian M. Touyz, Ernesto L. Schiffrin
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XV, 437 páginas) _b78 ilustraciones, 40 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1527
505	0		_aLarge Scale Transcriptome Analysis -- Methods to Assess Genetic Risk Prediction -- Microarray Analysis of Hypertension -- Tissue Proteomics in Vascular Disease -- Urine Metabolomics in Hypertension Research -- Systems Biology Approach in Hypertension Research -- Measurement of Angiotensin Peptides: HPLC, RIA -- Measurement of Angiotensin Converting Enzyme 2 Activity in Biological Fluid (ACE2) -- Determining the Enzymatic Activity of Angiotensin-Converting Enzyme 2 (ACE2) in Brain Tissue and Cerebrospinal Fluid Using a Quenched Fluorescent Substrate -- Measurement of Cardiac Angiotensin II by Immunoassays, HPLC-chip/Mass Spectrometry and Functional Assays -- Analysis of the Aldosterone Synthase (CYP11B2) and 11b-Hydroxylase (CYP11B1) Genes -- Dopaminergic Immunofluorescence Studies in Kidney Tissue -- Techniques for the Evaluation of the Genetic Expression, Intracellular Storage and Secretion of Polypeptide Hormones with Special Reference to the Natriuretic Peptides (Nps) -- Intracellular Free Calcium Measurement Using Confocal Imaging -- Measuring T-type Calcium Channel Currents in Isolated Vascular Smooth Muscle Cells -- In Vitro Analysis of Hypertensive Signal Transduction: Kinase Activation, Kinase Manipulation, and Physiologic Outputs -- In vitro and in vivo Approaches to Assess Rho-kinase Activity -- NADPH Oxidases and Measurement of Reactive Oxygen Species -- Measurement of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation -- Assessment of Caveolae/Lipid Rafts in Isolated Cells -- Isolation and Characterization of Circulating Microparticles by Flow Cytometry -- Isolation of Mature Adipocytes from White Adipose Tissue and Gene Expression Studies by Real-Time Quantitative RT-PCR -- Isolation and Differentiation of Murine Macrophages -- Isolation and Differentiation of Human Macrophages -- Isolation of Immune Cells for Adoptive Transfer -- Isolation and Culture of Endothelial Cells from Large Vessels -- Isolation and Culture of Vascular Smooth Muscle Cells from Small and Large Vessels -- Evaluation of Endothelial Dysfunction in vivo -- Vascular Reactive of Isolated Aorta To Study the Angiotensin-(1-7) Actions -- Generation of a Mouse Model with Smooth Muscle Cell Specific Loss of the Expression of< PPARγ -- Renal Delivery of Anti-microRNA Oligonucleotides in Rats -- In Vivo Analysis of Hypertension: Induction of Hypertension, In Vivo Kinase Manipulation And Assessment Of Physiologic Outputs. .
520			_aThis volume provides comprehensive protocols that researchers can use to dissect out fundamental principles and molecular mechanisms of hypertension, extending from genetics of experimental hypertension to biomarkers in clinical hypertension. Hypertension: Methods and Protocols guides readers through chapters on genetics and mmics of hypertension; the Renin-Angiotensin-Aldosterone System; vasoactive agents and hypertension; signal transduction and reactive oxygen species; novel cell models and approaches to study molecular mechanisms of hypertension; vascular physiology; and new approaches to manipulate mouse models to study molecular mechanisms of hypertension. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Hypertension: Methods and Protocols aims to ensure successful results in the further study of this vital field.
988			_aSpringer_Protocols_2017
650		7	_2embne _9140794 _aHipertensión _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493966233
776	0	8	_iPrinted edition: _z9781493966240
776	0	8	_iPrinted edition: _z9781493982530
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6625-7 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI