000			04972nam a2200397 i 4500
999			_c393637 _d393637
001			393637
003			ES-MaUEC
005			20231227121637.0
006			a|||| o|||| 00| 0
007			cr nn 008mamaa
008			150623s2015 xxua o |||| 0|eng d
020			_a9781493927159
024	7		_a10.1007/978-1-4939-2715-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH441 _b2015 EB
245	0	0	_aPyrosequencing : _bMethods and Protocols _cedited by Ulrich Lehmann, Jörg Tost
250			_a2nd edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XIV, 414 páginas) _b83 ilustraciones, 57 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1315
505	0		_aThe History of PyrosequencingÒ -- Pyro Mark® Instruments, Chemistry and Software for Pyrosequencing® Analysis -- Software-Based Pyrogram® Evaluation -- Quantitative Validation and Quality Control of Pyrosequencing® Assays -- Extended KRAS and NRAS Mutation Profiling by Pyrosequencing® -- Universal BRAF State Detection by the Pyrosequencing®-based U‑BRAFV600 Assay -- Pyrosequencing®-based Identification of Low Frequency Mutations Enriched through Enhanced-ice-COLD-PCR -- Analysis of Mutational Hotspots in Routinely Processed Bone Marrow Trephines by Pyrosequencing® -- Analysis of Copy Number Variation By Pyrosequencing® using Paralogous Sequences -- Prenatal Diagnosis of Chromosomal Aneuploidies by Quantitative Pyrosequencing® -- HLA-B and HLA-C Supratyping by Pyrosequencing® -- Allele Quantification Pyrosequencing® at Designated SNP sites to Detect Allelic Expression Imbalance and Loss-of-heterozygosity -- Quantitative DNA Methylation Analysis by Pyrosequencing® -- ­Quantitative Methylation Analysis of the PCDHB Gene Cluster -- Assessment of Changes in global DNA Methylation Levels by Global Analysis of DNA 5-methylcytosine using the Luminometric Methylation Assay, LUMA -- Limiting Dilution Bisulfite Pyrosequencing®: a Method for Methylation Analysis of Individual DNA Molecules in a Single or a Few Cells -- Detection of Loss of Imprinting by Pyrosequencing® -- Analysis of DNA Methylation Patterns in Single Blastocysts by Pyrosequencing® -- Allele-specific DNA methylation detection by Pyrosequencing® -- SNP-based Quantification of Allele-Specific DNA Methylation Patterns by Pyrosequencing® -- DNA Methylation Analysis of ChIP Products at Single Nucleotide Resolution byPyrosequencing® -- Multiplex Pyrosequencing®: Simultaneous Genotyping Based on SNPs from Distant Genomic Regions -- Detection of Drug-Resistant Mycobacterium tuberculosis -- Application of Pyrosequencing® in Food Biodefense -- Forensic Analysis of Mitochondrial and Autosomal Markers using Pyrosequencing® -- Tissue-specific DNA Methylation Patterns in Forensic Samples Detected By Pyrosequencing®.
520			_aThe primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols. Beginning with an up-to-date overview of the biochemistry, the volume continues with quantitative analysis of genetic variation, ratio of expressed alleles at the RNA level, analysis of DNA methylation, global DNA methylation assays, specialized applications for DNA methylation analysis including loss of imprinting, single blastocyst analysis, allele-specific DNA methylation patterns, DNA methylation patterns associated with specific histone modifications. The volume further details tools and protocols for the detection of viruses and bacteria, and genetic and epigenetic analyses for forensics using Pyrosequencing. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.   Comprehensive and adaptable, Pyrosequencing: Methods and Protocols, Second Edition will greatly aid doctorial students, postdoctoral investigators, and research scientists studying different aspects of genetics and cellular and molecular biology.
988			_aSpringer_Protocols_2015
650		7	_2embne _9138044 _aGenética _xTécnica _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493927142
776	0	8	_iPrinted edition: _z9781493927166
776	0	8	_iPrinted edition: _z9781493949090
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2715-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI