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003			ES-MaUEC
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007			cr nn 008mamaa
008			100301s1998 xxu| o |||| 0|eng d
020			_a9781592592579
024	7		_a10.1007/978-1-59259-257-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQR183 _b1998 EB
245	0	0	_aImmunochemical Protocols _cedited by John Pound
250			_a2nd edition 1998
264		1	_aTotowa, NJ _bHumana Press _c1998
300			_a1 recurso en línea (XIII, 508 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v80
505	0		_aProduction of Polyclonal Antisera -- Raising Polyclonal Antibodies Using Nitrocellulose-Bound Antigen -- Production and Characterization of Antibodies Against Synthetic Peptides -- Preparation and Testing of Monoclonal Antibodies to Recombinant Proteins -- A Simple Hollow-Fiber Bioreactor for the "In-House" Production of Monoclonal Antibodies -- Screening of Monoclonal Antibodies Using Antigens Labeled with Acetylcholinesterase -- Synthesis of Peptides for Use as Immunogens -- Use of Proteins Blotted to Polyvinylidene Difluoride Membranes as Immunogens -- Purification of Glycoproteins and Their Use as Immunogens -- IgG Purification -- Purification of Monoclonal Antibodies -- Production of Bispecif ic and Trispecific F(ab)2 and F(ab)3 Antibody Derivatives -- Preparation of Cytotoxic Antibody-Toxin Conjugates -- Immunoaffinity Purification and Quantification of Antibody-Toxin Conjugates -- Competitive ELISA -- Epitope Mapping -- Coupling of Antibodies with Biotin -- Preparation of Avidin Conjugates -- Enhanced Chemiluminescence Immunoassay -- Nonradioactive Methods for Visualization of Protein Blots -- Colloidal Gold Staining and Immunoprobing on the Same Western Blot -- Erasure of Western Blots After Autoradiographic or Chemiluminescent Detection -- Colloidal Gold Staining and Immunodetection in 2D Protein Mapping -- Enzyme-Antienzyme Method for Immunohistochemistry -- Immunohistochemical Detection of Bromodeoxyuridine-Labeled Nuclei for In Vivo Cell Kinetic Studies -- Cell Kinetic Studies Using a Monoclonal Antibody to Bromodeoxyuridine -- Immunohistochemical Detection of Cells in the Division Cycle Using Antibodies to Proliferating Cell Nuclear Antigen (PCNA) -- Double Label Immunohistochemistry on Tissue Sections Using Alkaline Phosphatase and Peroxidase Conjugates -- Preparation of Colloidal Gold Probes -- Immunogold Probes in Light Microscopy -- Immunogold Probes in Electron Microscopy -- Electron Microscopic-Silver Enhancement for Double Labeling with Antibodies Raised in the Same Species -- Flow Cytometric Analysis of Cell Surface Antigen Density -- Multiple Immunofluorescence Analysis of Cells Using Flow Cytometry -- Flow Cytometric Methods of Analyzing Apoptotic Cells -- Flow Cytometric Detection of Proliferation-Associated Antigens, PCNA and Ki-67 -- Sorting of Human Peripheral Blood T-Cell Subsets Using Immunomagnetic Beads -- Preparation and Use of Nonradioactive Hybridization Probes -- Cellular Human and Viral DNA Detection by Nonisotopic In Situ Hybridization -- Biotinylated Probes in Colony Hybridization -- Nonisotopic In Situ Hybridization -- Expression of Recombinant Antibody Fusion Proteins in E. coli -- Expression of Antibody Fusion Proteins in Mammalian Cells -- Antibody Screening of Bacteriophage ? gt-11 DNA Expression Libraries -- Display of Antibody Chains on Filamentous Bacteriophage -- Production of Human Fab Antibody Fragments from Phage Display Libraries -- Antibodies from Phage Display Libraries as Immunochemical Reagents.
520			_aThis much anticipated second edition of the best selling Immunological Protocols provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for ready use by molecular biologists. It covers the breadth of relevant established methods-from protein blotting and immunoassays through the visualization of cellular antigens and in situ hybridization-each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins, and their various conjugates. The second edition of Immunochemical Protocols brings many previously specialized techniques within the grasp of all laboratory workers. It remains, like its predecessor, "an excellent laboratory manual and reference" and opens the door to the productive use of a new generation of immunochemical reagents with exciting possibilities.
988			_aSpringer_Protocols_1998
650		7	_2embne _9138330 _aInmunología _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9780896034938
776	0	8	_iPrinted edition: _z9781489942456
776	0	8	_iPrinted edition: _z9780896033887
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59259-257-9 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
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998			_b12/2023 _dz _eb _zSI