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008			170601s2017 xxua o |||| 0|eng d
020			_a9781493970001
024	7		_a10.1007/978-1-4939-7000-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP551 _b2017 EB
245	0	0	_aProtein Crystallography : _bMethods and Protocols _cedited by Alexander Wlodawer, Zbigniew Dauter, Mariusz Jaskolski
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XIII, 672 páginas) _b173 ilustraciones, 145 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1607
505	0		_aExpression and Purification of Recombinant Proteins in Escherichia Coli with a His6 or Dual His6-MBP Tag -- Protein Crystallization -- Advanced Methods of Protein Crystallization -- The 'Sticky Patch' Model of Crystallization and Modification of Proteins for Enhanced Crystallizability -- Crystallization of Membrane Proteins: and Overview -- Locating and Visualizing Crystals for X-ray Diffraction Experiments -- Collection of X-ray Diffraction Data from Macromolecular Crystals -- Identifying and Overcoming Crystal Pathologies: Disorder and Twinning -- Applications of X-ray Micro-beam for Data Collection -- Serial Synchrotron X-ray Crystallography (SSX) -- Time-Resolved Macromolecular Crystallography at Modern X-ray Sources -- Structure Determination Using X-ray Free-electron Laser Pulses -- Processing of XFEL Data -- Many Ways to Derivatize Macromolecules and their Crystals for Phasing -- Experimental Phasing: Substructure Solution and Density Modification as Implemented in SHELX -- Contemporary Use of Anomalous Diffraction in Biomolecular Structure Analysis -- Long-Wavelength X-ray Diffraction and its Applications in Macromolecular Crystallography -- Acknowledging Errors: Advanced Molecular Replacement with Phaser -- Rosetta Structure Prediction as a Tool for Solving Difficult Molecular Replacement Problems -- Radiation Damage in Macromolecular Crystallography -- Boxes of Model Building and Visualization -- Structure Refinement at Atomic Resolution -- Low Resolution Refinement of Atomic Models against Crystallographic Data -- Stereochemistry and Validation of Macromolecular Structures -- Validation of Protein-Ligand Crystal Structure Models: Small Molecule and Peptide Ligands -- Protein Data Bank - the Single Global Macromolecular Structure Archive Managed by the Worldwide Protein Data Bank -- Databases, Repositories and Other Data Resources in Structural Biology.
520			_aThis volume provides methods for modern macromolecular crystallography, including all steps leading to crystal structure determination and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein Crystallography aims to ensure successful results in the further study of this vital field.
988			_aSpringer_Protocols_2017
650		7	_2embne _9139861 _aProteínas _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493969982
776	0	8	_iPrinted edition: _z9781493969999
776	0	8	_iPrinted edition: _z9781493983636
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7000-1 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI