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008			100301s2009 xxu| o |||| 0|eng d
020			_a9781603274838
024	7		_a10.1007/978-1-60327-483-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQL959 _b2009 EB
245	0	0	_aMolecular Embryology : _bMethods and Protocols _cedited by Paul Sharpe, Ivor Mason
250			_a2nd edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XVII, 798 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v461
505	0		_aThe Mouse Embryo -- The Mouse as a Developmental Model -- Culture of Postimplantation Mouse Embryos -- Organ Culture in the Analysis of Tissue Interactions -- Treatment of Mice with Retinoids In Vivo and In Vitro -- Analysis of Skeletal Ontogenesis through Differential Staining of Bone and Cartilage -- Cell Grafting and Labeling in Postimplantation Mouse Embryos -- Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs -- Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells -- Gene Trapping in Mouse Embryonic Stem Cells -- Application of lacZ Transgenic Mice to Cell Lineage Studies -- Transgenic RNA Interference to Investigate Gene Function in the Mouse -- Mouse Primordial Germ Cells -- Gene Transfer to the Rodent Embryo by Retroviral Vectors -- The Chicken Embryo -- The Avian Embryo -- Chick Embryos -- New Culture -- EC Culture -- Grafting Hensen's Node -- Grafting of Somites -- Microsurgical Manipulation of the Notochord -- Transplantation of Avian Neural Tissue -- Grafting of Apical Ridge and Polarizing Region -- Tissue Recombinations in Collagen Gels -- Quail-Chick Chimeras -- Using Fluorescent Dyes for Fate Mapping, Lineage Analysis, and Axon Tracing in the Chick Embryo -- Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses -- Electroporation in Avian Embryos -- Amphibian Embryos -- An Overview of Xenopus Development -- Mesoderm Induction Assays -- Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian -- A Method for Generating Transgenic Frog Embryos -- Axolotl/Newt -- Zebrafish And Medaka -- The Zebrafish -- Small-Scale Marker-Based Screening for Mutations in Zebrafish Development -- Microinjection and Cell Transplantation in Zebrafish Embryos -- Recent Advances in Meganuclease-and Transposon-Mediated Transgenesis of Medaka and Zebrafish -- Retinoids in Nonmammalian Embryos -- Nonvertebrate Chordates -- Protochordates -- Molecular Techniques -- Subtractive Hybridization and Construction of cDNA Libraries -- Differential Display of Eukaryotic mRNA -- Using DNA Microarrays -- Profiling Gene Transcription in the Developing Embryo -- RT-PCR on Embryos Using Degenerate Oligonucleotide Primers -- Single-Cell RT-PCR cDNA Subtraction -- In-Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections -- In-Situ Hybridization and Immunohistochemistry in Whole Embryos -- Wholemount In Situ Hybridization to Xenopus Embryos -- Wholemount In Situ Hybridization to Amphioxus Embryos -- In-Situ Hybridization to Sections (Nonradioactive) -- Immunohistochemistry Using Polyester Wax -- Immunohistochemistry on Whole Embryos -- Whole Embryo Assays for Programmed Cell Death -- Protein Techniques -- Microscopy and Photography -- Microscopy and Photomicrography Techniques.
520			_aIn Molecular Embryology: Methods and Protocols, Second Edition, expert investigators provide a comprehensive guide to the cutting-edge methods used across the dramatically growing field of vertebrate molecular embryology. Time-tested techniques take advantage of the most commonly used vertebrate experimental models: murine embryos for their genetics, chick embryos for in vivo manipulation, zebrafish for mutagenesis, amphibian embryos, and nonvertebrate chordates. The second edition collects classic protocols which have become standard techniques in the laboratory and presents them in a complementary fashion with novel and emerging approaches, allowing researcher to become more familiar with commonly studied embryos used in biomedical research. Insightful to the experienced professional, Molecular Embryology: Methods and Protocols, Second Edition, presents cutting-edge findings of perhaps the greatest period in growth and productivity in the field of developmental biology.
988			_aSpringer_Protocols_2009
650		7	_2embne _9140065 _aEmbriología _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781603279901
776	0	8	_iPrinted edition: _z9781617375651
776	0	8	_iPrinted edition: _z9781588294319
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-483-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI