000			03988nam a2200385 i 4500
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003			ES-MaUEC
005			20231227135858.0
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007			cr nn 008mamaa
008			100301s2008 xxu| o |||| 0|eng d
020			_a9781603272148
024	7		_a10.1007/978-1-60327-214-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH588 .S83 _b2008 EB
245	0	0	_aGermline Stem Cells _cedited by Steven X. Hou, Shree Ram Singh
250			_a1st edition 2008
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XII, 273 páginas)
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v450
505	0		_aIdentification and Regulation of Germline Stem Cells in Model Organisms -- The Development of Germline Stem Cells in Drosophila -- Analysis of the C. elegans Germline Stem Cell Region -- Immunohistological Techniques for Studying the Drosophila Male Germline Stem Cell -- Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells -- Structural Polarity and Dynamics of Male Germline Stem Cells in an Insect (Milkweed Bug Oncopeltus fasciatus) -- High-Resolution Light Microscopic Characterization of Spermatogonia -- Identification and Characterization of Spermatogonial Subtypes and Their Expansion in Whole Mounts and Tissue Sections from Primate Testes -- Epigenetic Control in Male Germ Cells -- GDNF Maintains Mouse Spermatogonial Stem Cells In Vivo and In Vitro -- In Vitro Culture and Applications of Germline Stem Cells -- Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts -- Spermatogonial Stem Cell Transplantation, Testicular Function, and Restoration of Male Fertility in Mice -- Isolating Highly Pure Rat Spermatogonial Stem Cells in Culture -- Deriving Mouse Spermatogonial Stem Cell Lines -- Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes -- Testicular Germ Cell Tumors in Mice -- Study Origin of Germ Cells and Formation of New Primary Follicles in Adult Human and Rat Ovaries.
520			_aThe knowledge gained by studying germline stem cells, the source of human and animal reproduction, may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in humans, and treating testicular and ovary tumors. In Germline Stem Cells, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize and maintain them. Divided into two parts, the volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells. Following the highly successful Methods in Molecular Biology™ series format, each chapter includes a brief introduction to the subject, a list of necessary equipment and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Germline Stem Cells is the perfect reference for scientists exploring this particular exciting and important subdivision in the fast-paced field of stem cell research.
988			_aSpringer_Protocols_2008
650		7	_2embne _9160528 _aCélulas madre
776	0	8	_iPrinted edition: _z9781617378805
776	0	8	_iPrinted edition: _z9781603272131
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-214-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI