000 04739nam a2200397 i 4500
999 _c393618
_d393618
001 393618
003 ES-MaUEC
005 20231227141748.0
006 a|||| o|||| 00| 0
007 cr nn 008mamaa
008 100301s2003 xxu| o |||| 0|eng d
020 _a9781592593323
024 7 _a10.1385/1592593321
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
_dES-MaUEC
050 4 _aRD94
_b2003 EB
245 0 0 _aWound Healing :
_bMethods and Protocols
_cedited by Luisa A. DiPietro, Aime L. Burns
250 _a1st edition 2003
264 1 _aTotowa, NJ
_bHumana Press
_c2003
300 _a1 recurso en línea (XVI, 468 páginas)
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Medicine
_x1940-6037
_v78
505 0 _aExperimental Models of Wound Healing -- Excisional Wound Healing -- Methods in Reepithelialization -- Incisional Wound Healing -- Animal Models of Ischemic Wound Healing -- Corneal Injury -- Subcutaneous Sponge Models -- A Mouse Model of Burn Wounding and Sepsis -- A Porcine Burn Model -- Wound Healing in Airways In Vivo -- Murine Models of Intestinal Anastomoses -- Murine Model of Peritoneal Adhesion Formation -- Methods for Investigating Fetal Tissue Repair -- Growth of Human Blood Vessels in Severe Combined Immunodeficient Mice -- Tissue Repair in Models of Diabetes Mellitus -- Wound Healing Studies in Transgenic and Knockout Mice -- Wound Repair in Aging -- Specimen Collection and Analysis -- Suction Blister Model of Wound Healing -- Implantable Wound Healing Models and the Determination of Subcutaneous Collagen Deposition in Expanded Polytetrafluoroethylene Implants -- The Fibroblast-Populated Collagen Lattice -- A Quantifiable In Vitro Model to Assess Effects of PAI-1 Gene Targeting on Epithelial Cell Motility -- Human Skin Organ Culture -- In Vitro Matrigel Angiogenesis Model -- Analysis and Manipulation of Wound Healing -- Quantification of Wound Angiogenesis -- In Vivo Matrigel Migration and Angiogenesis Assays -- Endothelial Cell Migration Assay -- Analysis of Collagen Synthesis -- Method for Detection and Quantitation of Leukocytes During Wound Healing -- Detection of Reactive Oxygen Intermediate Production by Macrophages -- Measurement of Chemokines at the Protein Level in Tissue -- Methods of Measuring Oxygen in Wounds -- Isolation, Culture, and Characterization of Human Intestinal Smooth Muscle Cells -- Use of High-Throughput Microarray Membranes for cDNA Analysis of Cutaneous Wound Repair -- Particle-Mediated Gene Therapy of Wounds.
520 _aDuring the past decade the significant progress made in cell and molecular biotechnology has led to exciting discoveries in the field of tissue repair, producing a wealth of information about the healing process. In Wound Healing: Methods and Protocols, expert researchers and physicians not only describe classic and contemporary laboratory methods for studying tissue repair using a broad range of wound healing models, but also provide sensitive assays for the assessment of tissue healing. The systems detailed include human, animal, in vitro, in vivo, and impaired healing models, as well as models in which underlying systemic and genetic conditions influence the healing process. Useful for the study of many biological processes, including angiogenesis, epidermal differentiation and repair, acute inflammation, ECM synthesis, and remodeling, these powerful models illustrate biochemical, molecular, and surgical techniques designed for the analysis and manipulation of the healing wound, and provide step-by-step instructions to ensure successful results. In many cases, several different approaches to a single process are examined, with sample results and analyses given to help users in selecting the approach most suited to the problem at hand. Comprehensive and enlightening, Wound Healing: Methods and Protocols offers both basic and clinical scientists a thought-provoking and highly practical collection of widely used model systems and methods for studying the injury repair process.
988 _aSpringer_Protocols_2003
650 7 _2embne
_9139586
_aHeridas y lesiones
_xModelos animales
_vManuales de laboratorio
776 0 8 _iPrinted edition:
_z9781617372964
776 0 8 _iPrinted edition:
_z9780896039995
776 0 8 _iPrinted edition:
_z9781489938343
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592593321
_zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942 _2lcc
_cLE
998 _b12/2023
_dz
_eb
_zSI