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008			151007s2015 xxua o |||| 0|eng d
020			_a9781493929382
024	7		_a10.1007/978-1-4939-2938-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQM567 _b2015 EB
245	0	0	_aCartilage Tissue Engineering : _bMethods and Protocols _cedited by Pauline M. Doran
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XI, 290 páginas) _b47 ilustraciones, 38 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1340
505	0		_aCartilage Tissue Engineering: What Have We Learned in Practice? -- Human Foetal and Adult Chondrocytes -- Mesenchymal Stem Cells Derived from Human Bone Marrow -- Mesenchymal Stem Cells Derived from Human Adipose Tissue -- Derivation and Chondrogenic Commitment of Human Embryonic Stem Cell-Derived Mesenchymal Progenitors -- Differentiation of Human Induced Pluripotent Stem Cells to Chondrocytes -- Gene Transfer and Gene Silencing in Stem Cells to Promote Chondrogenesis -- Hydrogels with Tunable Properties -- Decellularized Extracellular Matrix Scaffolds for Cartilage Regeneration -- Use of Interim Scaffolding and Neotissue Development to Produce a Scaffold-Free Living Hyaline Cartilage Graft -- Bioprinted Scaffolds for Cartilage Tissue Engineering -- Scaffolds for Controlled Release of Cartilage Growth Factors -- Nanostructured Capsules for Cartilage Tissue Engineering -- Stratified Scaffolds for Osteochondral Tissue Engineering -- Mechanobioreactors for Cartilage Tissue Engineering -- Shear and Compression Bioreactor for Cartilage Synthesis -- Microbioreactors for Cartilage Tissue Engineering -- Transplantation of Tissue-Engineered Cartilage in an Animal Model (Xenograft and Autograft): Construct Validation -- Proteomic Analysis of Engineered Cartilage -- Mechanical Testing of Cartilage Constructs.
520			_aThis volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; differentiation; synthesis and application of three-dimensional scaffolds; design and operation of bioreactors; in vivo testing of engineered constructs; and molecular and functional analysis of cartilage cells and tissues. Frequently used technologies are covered, as well as more recent advances in inspirational areas such as 'smart' biomaterial development, novel bioreactor design, -omics analysis, and genetic manipulation of matrix synthesis. The key procedures discussed either underpin the progress already achieved in cartilage tissue engineering or are indicative of the direction of future research in the area. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cartilage Tissue Engineering: Methods and Protocols is a useful resource that informs the scientific community about the experimental work covering a broad range of objectives for cartilage synthesis and regeneration. .
988			_aSpringer_Protocols_2015
650		7	_2embne _9679376 _aCartílago _vManuales de laboratorio
650		7	_2embne _9668849 _aTejidos (Biología) _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493929375
776	0	8	_iPrinted edition: _z9781493929399
776	0	8	_iPrinted edition: _z9781493948970
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2938-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b01/2024 _dz _eb _zSI