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008			110302s2011 xxua o |||| 0|eng d
020			_a9781617790188
024	7		_a10.1007/978-1-61779-018-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQH450.2 _b2011 EB
245	0	0	_aRNA and DNA Editing : _bMethods and Protocols _cedited by Ruslan Aphasizhev
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XIII, 275 páginas) _b57 ilustraciones, 1 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v718
505	0		_aThree-Dimensional Reconstruction of Trypanosoma brucei Editosomes Using Single-Particle Electron Microscopy -- iCODA: RNAi-Based Inducible Knock-In System in Trypanosoma brucei -- Perturbing A-to-I RNA Editing Using Genetics and Homologous Recombination -- Laser Microdissection and Pressure Catapulting of Single Human Motor Neurons for RNA Editing Analysis -- Biochemical Identification of A-to-I RNA Editing sites by the Inosine Chemical Erasing (ICE) Method -- Identifying mRNA Editing Deaminase Targets by RNA-Seq -- Mouse and Other Rodent Models of C to U RNA Editing -- In vivo Analysis of RNA Editing in Plastids -- Identifying Specific Trans-Factors of RNA Editing in Plant Mitochondria by Multiplex Single Base Extension Typing -- Complementation of Mutants in Plant Mitochondrial RNA Editing by Protoplast Transfection -- A High Throughput Assay for DNA Deaminases -- Biochemical Fractionation and Purification of High-Molecular-Mass APOBEC3G Complexes -- Analysis of tRNA Editing in Native and Synthetic Substrates -- Post-Transcriptional Modification of RNAs by Artificial Box H/ACA and Box C/D RNPs -- Functional Analysis of Non-Coding RNAs in Trypanosomes: RNA Walk, A Novel Approach to Study RNA-RNA Interactions Between Small RNA and Its Target -- A Post-Labeling Approach for the Characterization and Quantification of RNA Modifications Based on Site-Directed Cleavage by DNAzymes.
520			_aThe recent expansion in diversity of RNA and DNA editing types has stimulated the development of many unique genetic, molecular, biochemical, and computational approaches to biological issues.  In RNA and DNA Editing: Methods and Protocols, leading experts in the field introduce methods developed over the last few years to study editing substrates, mechanisms of specificity, and functions of RNA and DNA editing enzymes and complexes. Sections of the book are dedicated to state-of-the art techniques which enable investigation of uracil insertion/deletion RNA editing in mitochondrion of Trypanosoma brucei, adenosine to inosine RNA editing, cytidine to uracil RNA and DNA editing, as well as tRNA editing and RNA modifications.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, RNA and DNA Editing: Methods and Protocols seeks to inspire the further development of these vital and powerful techniques.
988			_aSpringer_Protocols_2011
650		7	_2embne _9147235 _aTranscripción genética _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781617790171
776	0	8	_iPrinted edition: _z9781617790195
776	0	8	_iPrinted edition: _z9781493958214
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-018-8 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI