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008			140428s2014 xxua o |||| 0|eng d
020			_a9781493906062
024	7		_a10.1007/978-1-4939-0606-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQK881 _b2014 EB
245	0	0	_aPlant Isoprenoids : _bMethods and Protocols _cedited by Manuel Rodríguez-Concepción
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (XI, 303 páginas) _b53 ilustraciones, 12 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1153
505	0		_aPlant Isoprenoids: A General Overview -- Measuring the Activity of 1-Deoxy-D-Xylulose 5-Phosphate Synthase, the First Enzyme in the MEP Pathway, in Plant Extracts -- Determination of 3-Hydroxy-3-methylglutaryl CoA Reductase Activity in Plants -- Farnesyl Diphosphate Synthase Assay -- Metabolite Profiling of Plastidial Deoxyxylulose-5-Phosphate Pathway Intermediates by Liquid Chromatography and Mass Spectrometry -- Analysis of Carotenoids and Tocopherols in Plant Matrices and Assessment of Their In Vitro Antioxidant Capacity -- Simultaneous Analyses of Oxidized and Reduced Forms of Photosynthetic Quinones by High-Performance Liquid Chromatography -- Determination of Sterol Lipids in Plant Tissues by Gas Chromatography and Q-TOF Mass Spectrometry -- Analysis of Plant Polyisoprenoids -- Analysis of Diterpenes and Triterpenes from Plant Foliage and Roots -- Gas Chromatography Mass-Spectrometry Method for Determination of Biogenic Volatile Organic Compounds Emitted by Plants -- Analysis of Steroidal Alkaloids and Saponins in Solanaceae Plant Extracts using UPLC-qTOF Mass Spectrometry -- Isoprenoid and Metabolite Profiling of Plant Trichomes -- Sample Preparation for Single Cell Transcriptomics - Essential Oil Glands in Citrus Fruit Peel as an Example -- Prenylquinone Profiling in Whole Leaves and Chloroplast Subfractions -- Confocal Laser Scanning Microscopy Detection of Chlorophylls and Carotenoids in Chloroplasts and Chromoplasts of Tomato Fruit -- Heterologous Expression of Triterpene Biosynthetic Genes in Yeast and Subsequent Metabolite Identification Through GC-MS -- High Throughput Testing of Terpenoid Biosynthesis Candidate Genes Using Transient Expression in Nicotiana benthamiana -- Heterologous Stable Expression of Terpenoid Biosynthetic Genes Using the Moss Physcomitrella patens -- Quantification of Plant Resistance to Isoprenoid Biosynthesis Inhibitors -- A Flexible Protocol for Targeted Gene Co-Expression Network Analysis.
520			_aPlant Isoprenoids: Methods and Protocols is a collection of detailed techniques that will be a useful tool for a wide range of plant biologists, as well as for scientists of other fields interested in plant isoprenoids. Isoprenoids are an incredibly diverse family and they participate in a large variety of processes. Divided into four convenient sections to better cover strategic areas in plant isoprenoid research, topics include measurement of core enzyme activities involved in the production of isoprenoid precursors, targeted analysis of major groups of isoprenoid metabolites, isoprenoid profiling in specialized organs such as trichomes and oil glands, as well as genetic, pharmacological, and bioinformatic tools that are particularly useful for plant molecular biologists. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible, Plant Isoprenoids: Methods and Protocols will serve as an excellent reference material that can be adapted to develop customized methods for different needs making the world of plant isoprenoids more accessible for all researchers.
988			_aSpringer_Protocols_2014
650		7	_2embne _9168920 _aPlantas _xMetabolismo _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781493906079
776	0	8	_iPrinted edition: _z9781493906055
776	0	8	_iPrinted edition: _z9781493953912
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0606-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zPRE