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008			100721s2010 xxua o |||| 0|eng d
020			_a9781603271752
024	7		_a10.1007/978-1-60327-175-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP772 .I5 _b2010 EB
245	0	0	_aInositol Phosphates and Lipids : _bMethods and Protocols _cedited by Christopher J. Barker
250			_a1st edition 2010
264		1	_aTotowa, NJ _bHumana Press _c2010
300			_a1 recurso en línea (X, 230 páginas) _b54 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v645
505	0		_aThe Role of Inositol and the Principles of Labelling, Extraction, and Analysis of Inositides in Mammalian Cells -- HPLC Separation of Inositol Polyphosphates -- Molecular Manipulation and Analysis of Inositol Phosphate and Pyrophosphate Levels in Mammalian Cells -- A Femtomole-Sensitivity Mass Assay for Inositol Hexakisphosphate -- Synthesis of InsP 7 by the Inositol Hexakisphosphate Kinase 1 (IP6K1) -- Protein Pyrophosphorylation by Diphosphoinositol Pentakisphosphate (InsP 7) -- Synthesis and Nonradioactive Micro-analysis of Diphosphoinositol Phosphates by HPLC with Postcolumn Complexometry -- Diphosphosinositol Polyphosphates and Energy Metabolism: Assay for ATP/ADP Ratio -- Isolation of Inositol 1,4,5-Trisphosphate Receptor-Associating Proteins and Selective Knockdown Using RNA Interference -- Phosphoinositide-Specific Phospholipase C ?1 Signal Transduction in the Nucleus -- Methods to Assess Changes in the Pattern of Nuclear Phosphoinositides -- Measurement of Phosphoinositide 3-Kinase Products in Cultured Mammalian Cells by HPLC -- Phosphoinositide Analysis by Liquid Chromatography-Mass Spectrometry -- Imaging Phosphoinositide Dynamics in Living Cells.
520			_aInositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In Inositol Phosphates and Lipids: Methods and Protocols, expert researchers introduce the basic methodological tools to measure inositol lipids and phosphates and also describe new approaches that have become available in the last 10 years, including RNA-silencing and the use of fluorescently labeled PH-domains to measure inositides in real-time in live cells, new sensitive methods to measure mass of both phosphates and lipids, as well as protocols involving inositol pyrophosphates. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, detailing tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Inositol Phosphates and Lipids: Methods and Protocols compiles many of the techniques that underscore phosphorylated inositol cell biology in one convenient guide.
988			_aSpringer_Protocols_2010
650		7	_2embne _9480129 _aInositol _vManuales de laboratorio
776	0	8	_iPrinted edition: _z9781607616795
776	0	8	_iPrinted edition: _z9781603271745
776	0	8	_iPrinted edition: _z9781617796890
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-175-2 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dq _eb _zSI