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008			150925s2016 xxua o |||| 0|eng d
020			_a9781493928125
024	7		_a10.1007/978-1-4939-2812-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC _dES-MaUEC
050		4	_aQP364.7 _b2016 EB
245	0	0	_aIonotropic Glutamate Receptor Technologies _cedited by Gabriela K. Popescu
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XII, 304 páginas) _b70 ilustraciones, 47 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v106
505	0		_aAssaying AMPA Receptor Oligomerization -- A Step-by-Step Guide to Single Subunit Counting Of Membrane-Bound Proteins in Mammalian Cells -- Counting NMDA Receptors at the Cell Surface -- Electron Microscopy Analysis of AMPA receptors in Dendritic Spines -- Electrophysiological Tagging of Ionotropic Receptors -- Functional Detection of Novel Triheteromeric NMDA Receptors -- Calcium Imaging to Study NMDA Receptor-mediated Cellular Responses -- NMR Approaches to Functional Dynamics Of Genetically Separated iGluR Domains -- Computing Conformational Free Energies of iGluR Ligand-Binding Domains -- LRET Methods for Investigating Conformational Changes in Functional Ionotropic Glutamate Receptors -- Assaying the Energetics of NMDA Receptor Pore Opening -- Constructing a Rapid Solution Exchange System -- Assessing Effects of Ligand-Binding Mutations To AMPA And Kainate Receptor Kinetics -- Analysis of Whole-Cell NMDA Receptor Responses -- Expression, Purification and Crystallization of Full Length Ionotropic Glutamate Receptors -- Timing AMPA Receptor Activation with Laser-pulse Photolysis -- Current Recording and Kinetic Analyses for Single AMPA Receptors -- Extracting Rate Constants for NMDA Receptor Gating from One-channel Current Recordings.
520			_aThis detailed volume explores key technologies that are used currently to investigate iGluR structure, function, and physiology.  Chapters in this book cover methods to help illuminate the assembly, trafficking, molecular composition, and subcellular location of iGluRs;   approaches used to understand the atomic organization of iGluRs; and techniques to monitor receptor activity in real time. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.  Concise and easy-to-use, Ionotropic Glutamate Receptor Technologies aims to facilitate the implementation of specific methods to iGluR investigations.
988			_aSpringer_Protocols_2016
650		7	_2embne _9147745 _aNeurotransmisores
776	0	8	_iPrinted edition: _z9781493928118
776	0	8	_iPrinted edition: _z9781493928132
776	0	8	_iPrinted edition: _z9781493948956
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2812-5 _zAcceso a este recurso digital (usuarios Universidad Europea de Madrid)
942			_2lcc _cLE
998			_b12/2023 _dz _eb _zSI